Method for preparing recombinant proteins through reduction of rnpA gene expression
AssigneeMedicosbiotech, Inc.
InventorsSang Yup Lee, Hannah Chung
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Abstract
The present invention relates to a method for improving the production of a difficult-to-express recombinant protein in a recombinant microorganism, and more particularly to a method for improving the production of a difficult-to-express recombinant protein by use of a recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced. According to the present invention, expressions of a large recombinant protein, a difficult-to-express protein and a useful protein can be dramatically increased by reducing expression of the rnpA gene.
Claims10
A recombinant vector for expressing a target protein, which comprises a gene encoding the target protein and an sRNA against a gene encoding ribonuclease P, wherein the target protein is a difficult-to-express protein.
A recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced, wherein the target protein is a difficult-to-express protein.
Dependencies are inferred from the claim text.