Nucleic acid-guided ordered protein assemblies and methods

A method for generating a nucleic acid-anchored ordered protein assembly (NOPA), the method comprising the steps of:

• (i) providing a nucleic acid-guided protein localization (NPL) array comprising a plurality of single stranded protein localization oligonucleotides (PLOs) linked at a plurality of positions to a solid support substrate surface, wherein the plurality of PLOs comprise at least a first position-linked PLO comprising a first protein localization (PL) sequence and a second position-linked PLO comprising a second PL sequence;
• (ii) contacting the nucleic acid array with at least a first nucleic acid anchor (NAA) protein and a second NAA protein, wherein the first NAA is linked to an anchoring oligonucleotide comprising an anchoring sequence complementary to the first PL sequence, and the second NAA protein is linked to an anchoring oligonucleotide (AO) comprising an anchoring sequence complementary to the second PL sequence;
• and
• (iii) hybridizing, under protein compatible conditions, the AOs of the first and second NAA proteins with the first and second PLOs, whereby the at least first and second NAA proteins are localized to the first and second locations to form an ordered protein assembly.