Methods for making definitive endoderm using at least GDF-8
AssigneeJanssen Biotech, Inc.
InventorsJanet Davis, Jiajian Liu, Christine Parmenter, Pascal Ghislain Andre Bonnet
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Abstract
The present invention is directed to methods to differentiate pluripotent stem cells. In particular, the present invention is directed to methods and compositions to differentiate pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage comprising culturing the pluripotent stem cells in medium comprising a sufficient amount of GDF-8 to cause the differentiation of the pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage.
Claims19
A method to differentiate pluripotent stem cells into definitive endoderm cells comprising culturing the pluripotent stem cells with a medium lacking activin A, and supplemented with GDF-8 and 14-Methyl-3,5,7,14,18,24,28-heptaazatetracyclo[20.3.1.1˜2,6˜.1˜8,12˜]octacosa-1(26),2(28),3,5,8(27),9,11,22,24-nonaen-17-one.
Dependencies are inferred from the claim text.