Point-of-care and/or portable platform for gene therapy

A method performed by a device, the method comprising:

• receiving, by a treatment chamber, a subject sample
• transferring, by at least one valve and at least one pump, the subject sample from the treatment chamber to at least one target cell selector;
• separating, by the at least one target cell selector, target cells from non-target cells in the subject sample by allowing the non-target cells to pass into a waste bag and retaining the target cells within the at least one target cell selector;
• transferring, by the at least one valve and the at least one pump, the target cells into the treatment chamber;
• introducing a genetic modifier to the target cells to generate genetically-modified target cells;
• pelleting the genetically-modified target cells within the treatment chamber through centrifugation to create a pelleted cell suspension;
• removing a specified supernatant volume from the pelleted cell suspension in the treatment chamber through a first tubing set connected to the treatment chamber;
• diluting the pelleted cell suspension with final formulation media comprising a pharmaceutically acceptable carrier;
• centrifuging the diluted pelleted cell suspension and removing an additional volume of supernatant in the treatment chamber through the first tubing set to form a final cell product formulation;
• and
• transferring the final cell product formulation into one or more cryobags through a second tubing set connected to the treatment chamber wherein the final cell product formulation is ready for administration to a subject upon completion of transfer into the one or more cryobags.