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US 10,350,147 B2Granted
Enamel products and methods of use
AssigneeThe Research Foundation for The State University of New York
InventorsStephanos Kyrkanides, Li Ma, Sabine Brouxhon
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Abstract
Described herein are three-dimensional (3D) culture systems that can be used to produce enamel organoids that generate enamel products. The invention features methods of culturing a variety of cell types to produce such enamel organoids; the organoids themselves; enamel products generated by the organoids; and methods of fashioning the enamel products into surgical restorations, including dental restorations and other prostheses.
Claims8
A method of generating an enamel product in vitro, the method comprising:
- (a) providing an oral epithelial keratinocyte in cell culture;
- (b) exposing the oral epithelial keratinocyte to a transforming growth factor β (TGFβ), thereby producing a dedifferentiated cell;
- (c) exposing the dedifferentiated cell to a serotonin receptor agonist, thereby producing an ameloblast-like cell that produces an extracellular matrix comprising amelogenin;
- and
- (d) exposing the extracellular matrix to a composition that induces mineralization, thereby producing an enamel product.
2 more elements
Dependencies are inferred from the claim text.