System and method for a biomimetic fluid processing

A method for generating a biological substance comprising:

• introducing a first biological composition into a first channel of a biomimetic microfluidic system at a first channel flow rate, the at least one first biological composition including a biological source material capable of producing a target biological substance;
• introducing a second biological composition into a second channel of the biomimetic microfluidic system at a second channel flow rate;
• selectively capturing, by a series of microchannels forming a fluid communication path between the first channel and the second channel, the biological source material;
• creating a differential between the channels to generate physiological shear rates along the second channel that induce the captured biological source material to produce the target biological substance;
• and
• retrieving, using the second biological composition, the produced target biological substance for harvesting.

A method for generating platelets comprising:

• introducing a first biological composition into a first channel of a biomimetic microfluidic system at a first channel flow rate, the first biological composition including megakaryocytes (MKs) capable of generating platelets (PLTs);
• introducing a second biological composition into a second channel of the biomimetic microfluidic system at a second channel flow rate;
• selectively capturing, in the first channel, by a series of microchannels forming a fluid communication path between the first channel and the second channel, one or more of the MKs;
• permitting proplatelets (“proPLTs”) formed from the one or more MKs to extend through the microchannels into the second channel;
• creating a differential between the channels to generate physiological shear rates along the second channel that induce production of the PLTs from the proPLTs;
• and
• retrieving, using the second biological composition, the produced PLTs.