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US 10,337,054 B2Granted
Enrichment of nucleic acid targets
AssigneeQuantum-Si Incorporated
InventorsAndrea Marziali, Milenko Despotovic, Joel Pel
Life of this patent
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Abstract
Methods and apparatus providing for the isolation of an unknown mutation from a sample comprising wild type nucleic acids and mutated nucleic acids through the application of time-varying driving fields and periodically varying mobility-altering fields to the sample within in an affinity matrix.
Claims19
A method for isolating a second molecule from a first molecule in a sample, the method comprising:
- applying a time-varying driving field to the sample in the presence of an affinity matrix comprising immobilized probes, the probes having a first binding affinity for the first molecule that is greater than a second binding affinity for the second molecule;
- applying a periodically varying, mobility-altering field that modifies the first and second binding affinities;
- wherein application of the time-varying driving field and the periodically varying, mobility-altering field concentrates the second molecule within the affinity matrix and separate from the first molecule;
- wherein the sample further comprises a background molecule with a third binding affinity that is less than the first and second binding affinities and wherein application of the time-varying driving field and periodically varying mobility-altering field concentrates the second molecule within the affinity matrix and separate from the first molecule and the background molecule.
1 more element
A method for isolating a second molecule from a first molecule in a sample, the method comprising:
- applying a time-varying driving field to the sample in the presence of an affinity matrix comprising immobilized probes, the probes having a first binding affinity for the first molecule that is greater than a second binding affinity for the second molecule;
- applying a periodically varying, mobility-altering field that modifies the first and second binding affinities;
- wherein application of the time-varying driving field and the periodically varying, mobility-altering field concentrates the second molecule within the affinity matrix and separate from the first molecule;
- wherein the first molecule is a wild type nucleic acid and the second molecule comprises a mutant nucleic acid.
1 more element
A method for isolating a second molecule from a first molecule in a sample, the method comprising:
- applying a time-varying driving field to the sample in the presence of an affinity matrix comprising immobilized probes, the probes having a first binding affinity for the first molecule that is greater than a second binding affinity for the second molecule;
- applying a periodically varying, mobility-altering field that modifies the first and second binding affinities;
- wherein application of the time-varying driving field and the periodically varying, mobility-altering field concentrates the second molecule within the affinity matrix and separate from the first molecule;
- wherein the sample comprises issue, blood, sputum, sweat, urine, feces, tears, aspirate, or a combination thereof.
1 more element
A method for isolating a second molecule from a first molecule in a sample, the method comprising:
- applying a time-varying driving field to the sample in the presence of an affinity matrix comprising immobilized probes, the probes having a first binding affinity for the first molecule that is greater than a second binding affinity for the second molecule;
- applying a periodically varying, mobility-altering field that modifies the first and second binding affinities;
- wherein application of the time-varying driving field and the periodically varying, mobility-altering field concentrates the second molecule within the affinity matrix and separate from the first molecule;
- wherein the affinity matrix comprises two or more different immobilized probes configured to work simultaneously and wherein application of the time-varying driving field and the periodically varying, mobility-altering field simultaneously concentrates a plurality of different target molecules from the sample.
1 more element
Dependencies are inferred from the claim text.