Labeling hydroxymethylated residues
AssigneesChildren's Medical Center Corporation, The Untted States of America AS Represented, By the Secretary, Department of Health and Human Services
InventorsAnjana Rao, Mamta Tahiliani, Kian Peng Koh, Suneet Agarwal, Aravind Iyer
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Abstract
The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.
Claims7
A method comprising:
- (a) labeling covalently, a hydroxyl group on a hydroxymethylated residue in a mammalian nucleic acid to generate a labeled 5-hydroxymethylcytosine residue, wherein said labeling comprises glycosylating the hydroxyl group by employing an alpha-glucosyltransferase, a beta-glucosyltransferase, or a beta-glucosyl-alpha-glucosyl-transferase;
- and
- (b) sequencing said mammalian nucleic acid comprising said labeled hydroxymethylated residue.
Dependencies are inferred from the claim text.