Kanamycin compound, kanamycin-producing Streptomyces species bacterium, and method of producing kanamycin
AssigneeIntron Biotechnology, Inc.
InventorsYeo Joon Yoon, Sung Ryeol Park, Je Won Park, Jae Kyung Sohng
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Abstract
Vectors expressing kanA-kanB-kanK and other kanamycin production-related genes, Streptomyces species recombinant bacteria transformed with the vectors, a method of producing kanamycin antibiotics by the bacteria, and a new kanamycin compound produced by the bacterium are provided. With the use of the recombinant bacteria of the present invention, the direct fermentative biosynthesis of amikacin and tobramycin as semi-synthetic kanamycins is possible, and the yield of kanamycin B as a precursor of the semi-synthetic kanamycin is improved.
Claims11
A vector for producing 1-N-AHBA-kanamycin comprising a gene set of
- kanA, kanB, kanK, kanF, kacA, kanE, kanC, kanD, btrG, btrH, btrI btrJ, btrK, btrO, and btrV.
A Streptomyces species bacterium for producing 1-N-AHBA-kanamycin, expressing a gene set of kanA, kanB, kanK, kanF, kacA, kanE, kanC, kanD, btrG, btrH, btrl, btrJ, btrK, btrO, and btrV.
A Streptomyces venezuelaeKCTC11727BP expressing a gene set of kanA, kanB, kanK, kanF, kacA, kanE, kanC, kanD, btrG, btrH, btrl, btrJ, btrK, btrO, and BtrV.
Dependencies are inferred from the claim text.