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US 10,337,031 B2Granted
Production of fragrant compounds
AssigneeFirmenich SA
InventorsMichel Schalk, Fabienne Deguerry, Pan Li, Xiufeng He, Qi Wang
Life of this patent
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Abstract
Provided herein is an isolated polypeptide from Juniperus virginiana, Platycladus orientalis ‘Beverleyensis’ or Platycladus orientalis comprising a (+)-cedrol or a (−)-thujopsene synthase. Further provided herein is an isolated nucleic acid molecule from Juniperus virginiana, Platycladus orientalis ‘Beverleyensis’ or Platycladus orientalis encoding a (+)-cedrol or (−)-thujopsene synthase. Further provided herein are methods of producing (+)-cedrol or (−)-thujopsene.
Claims11
A method of producing one or more sesquiterpenes comprising (+)-cedrol and/or (−)-thujopsene, the method comprising:
- a. contacting an acyclic farnesyl diphosphate (FPP) precursor with a polypeptide having a (+)-cedrol synthase activity and/or a (−)-thujopsene synthase activity wherein the polypeptide comprises:
- i. a sequence of amino acids that has at least 90% sequence identity to a polypeptide selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:
- 13 and SEQ ID NO:
- 14;
- or
- ii. a sequence of amino acids selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:
- 13 and SEQ ID NO:
- 14,
- to produce one or more sesquiterpenes comprising (+)-cedrol and/or (−)-thujopsene;
- and
- b. optionally isolating the (+)-cedrol and/or (−)-thujopsene.
8 more elements
Dependencies are inferred from the claim text.