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US 10,336,999 B2Granted
Compositions and methods for activating silent gene clusters
AssigneeZymergen Inc.
InventorsOliver Liu, Jeffrey Hoon Kim
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Abstract
The disclosure provides compositions and methods for producing natural products in microorganisms that are otherwise unexpressed, poorly expressed or poorly transcribed. In particular aspects, the disclosure provides compositions and methods for activating a silent gene or gene cluster with a bacteriophage and/or Streptomyces Antibiotic Regulatory Protein (SARP) transcription factor.
Claims10
A method of activating one or more native genes in each bacterial cell in a population of bacterial cells, the method comprising:
- infecting the population of bacterial cells with a plurality of recombinant bacteriophage, wherein
- (a) the bacteriophage are selected from a collection of recombinant phage comprising different heterologous nucleotide sequences that encode one or more bacterial transcription factors, and
- (b) each bacterial cell expresses the one or more native genes because of the expression of the one or more bacterial transcription factors;
- wherein the one or more native genes are transcriptionally silent, poorly expressed, or poorly transcribed in a control bacterial cell that has not been infected with the bacteriophage comprising the different heterologous nucleotide sequences encoding the one or more transcription factors;
- and wherein the population of bacteriophage-infected bacterial cells express different sets of heterologous transcription factors.
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Dependencies are inferred from the claim text.