Helicobacter pylori α-1,3 fucosyltransferase gene and protein with improved soluble protein expression and activity, and thereof application for synthesis of α-1,3 fucosyloligosaccharide

An α-1,3 fucosyltransferase mutant having any one sequence selected from (a) to (l):

• (a) a sequence wherein the amino acid at position 128 of any one of SEQ ID NOS:
• 1 to 3 is substituted with asparagine;
• (b) a sequence wherein the amino acid at position 129 of any one of SEQ ID NOS:
• 1 to 3 is substituted with glutamic acid;
• (c) a sequence wherein the amino acid at position 132 of any one of SEQ ID NOS:
• 1 to 3 is substituted with isoleucine;
• (d) a sequence wherein the amino acid at position 46 of any one of SEQ ID NOS:
• 1 to 3 is substituted with phenylalanine;
• (e) a sequence wherein the amino acid at position 128 of any one of SEQ ID NOS:
• 1 to 3 is substituted with asparagine and the amino acid at position 129 is substituted with glutamic acid;
• (f) a sequence wherein the amino acid at position 128 of any one of SEQ ID NOS:
• 1 to 3 is substituted with asparagine and the amino acid at position 132 is substituted with isoleucine;
• (g) a sequence wherein the amino acid at position 128 of any one of SEQ ID NOS:
• 1 to 3 is substituted with asparagine and the amino acid at position 46 is substituted with phenylalanine;
• (h) a sequence wherein the amino acid at position 128 of any one of SEQ ID NOS:
• 1 to 3 is substituted with asparagine, the amino acid at position 129 is substituted with glutamic acid, and the amino acid at position 132 is substituted with isoleucine;
• (i) a sequence wherein the amino acid at position 128 of any one of SEQ ID NOS:
• 1 to 3 is substituted with asparagine, the amino acid at position 129 is substituted with glutamic acid, and the amino acid at position 46 is substituted with phenylalanine;
• (j) a sequence wherein the amino acid at position 128 of any one of SEQ ID NOS:
• 1 to 3 is substituted with asparagine, the amino acid at position 132 is substituted with isoleucine, and the amino acid at position 46 is substituted with phenylalanine;
• (k) a sequence wherein the amino acid at position 128 of any one of SEQ ID NOS:
• 1 to 3 is substituted with asparagine, the amino acid at position 129 is substituted with glutamic acid, the amino acid at position 132 is substituted with isoleucine, and the amino acid at position 46 is substituted with phenylalanine;
• and
• (l) a sequence wherein the amino acid at one or more positions selected from the group consisting of positions 128, 129, 132 and 46 of any one of SEQ ID NOS:
• 1 to 3 is substituted with another amino acid, wherein the amino acid at position 128 is substituted with asparagine, the amino acid at position 129 is substituted with glutamic acid, the amino acid at position 132 is substituted with isoleucine, and the amino acid at position 46 is substituted with phenylalanine.