Methods for purification of a virus produced in vitro and clearance assay for the virus
AssigneeGrifols, S.A.
InventorsBrett Buno, Terri Journigan, Joann Hotta, Michael Burdick
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Abstract
A method of purification of non-enveloped or pseudo-enveloped virus produced in vitro uses a composition with at least one detergent. A method of purification can use multiple detergents, and a method of determining the presence and/or level of a non-enveloped or pseudo-enveloped virus in a sample includes treating with at least one detergent.
Claims22
A method of purifying hepatitis E virus (HEV) virus propagated in cell culture, the method comprising treating a sample comprising the HEV with a detergent, and purifying the HEV by both centrifugation or ultra-centrifugation and filtration or ultrafiltration, wherein the detergent is a detergent mixture comprising a combination of Triton X-100 and Lithium Dodecyl Sulfate.
A method of measuring a level of HEV in a sample, the method comprising the steps of:
- a) providing the sample to a mixture comprising a cell line and a culture medium;
- b) incubating the sample to allow propagation of the HEV, if present in the sample, to obtain an incubated portion;
- c) treating the incubated portion with a combination of Triton X-100 and Lithium Dodecyl Sulfate, to obtain a treated portion;
- d) collecting a part of the treated portion, to obtain a collected portion;
- and
- e) measuring the level of the HEV in the collected portion.
Dependencies are inferred from the claim text.