Assay for anti-polyvinyl alcohol antibodies
AssigneeAutoimmune Technologies, LLC
InventorsRussell B. Wilson, Kevin David Simpson
Life of this patent
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Abstract
An assay method for detecting antipolyvinyl alcohol (anti-PVAL) antibodies in test samples, such as serum, is described. The method comprises combining a sample to be tested for an anti-PVAL antibody with polyvinyl alcohol (PVAL) to form a binary complex of the PVAL with an anti-PVAL antibody in the sample, followed by reacting an indicator reagent with the binary complex to form a labeled ternary complex, and then detecting the presence or absence of the ternary complex in the sample. Kits for performing the test are also described.
Claims11
A method of detecting antipolyvinyl alcohol (anti-PVAL) antibody in a human bodily fluid sample, comprising the sequential steps of:
- (a) combining the human bodily fluid sample to be tested for an anti-PVAL antibody with polyvinyl alcohol (PVAL) bound to a solid support under conditions sufficient for the formation of specific binary complex between the PVAL bound to the solid support and an anti-PVAL antibody if present in the sample;
- (b) reacting an indicator reagent that is specific for human antibodies with the binary complex to form a labeled ternary complex;
- wherein the indicator reagent comprises a specific binding member conjugated to a detectable label, wherein the specific binding member is specific for human antibodies;
- (c) washing away any of the sample and the indicator reagent that is not bound to the solid support;
- and
- d) detecting the presence or absence of the anti-PVAL antibody in the sample by detecting the presence or absence of the detectable label on the solid support.
Dependencies are inferred from the claim text.