Compositions and methods for detection and discrimination of emerging influenza virus subtypes
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsBo Shu, Stephen Lindstrom
Life of this patent
Estimating the term…
Abstract
Compositions and methods for detecting presence of an emerging influenza virus in a sample, such as a biological sample obtained from a subject or an environmental sample, are disclosed. In some embodiments, the compositions and methods can be used to quickly identify particular subtypes of influenza virus (such as a pandemic and/or emerging influenza virus subtype). Probes and primers are provided herein that permit the rapid detection and/or discrimination of pandemic influenza virus subtype nucleic acids in a sample. Devices (such as arrays) and kits for detection and/or discrimination of influenza virus subtype nucleic acids are also provided.
Claims17
A kit for detecting an influenza virus nucleic acid molecule in a sample, comprising:
- a probe consisting of the nucleic acid sequence of SEQ ID NO:
- 3 and at least one detectable label attached to the probe, wherein the detectable label comprises a radioactive isotope, enzyme substrate, co-factor, ligand, chemiluminescent agent, fluorophore, fluorescence quencher, hapten, enzyme, chemical, or combinations thereof;
- and
- a forward primer consisting of SEQ ID NO:
- 1;
- and
- a reverse primer consisting of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.