Methods and materials for detecting genetic or epigenetic elements
AssigneeCascade Biosystems, Inc.
InventorsKenneth D. Smith, Nina Yazvenko, Mariya Smit
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Abstract
This document provides methods and materials for detecting genetic and/or epigenetic elements. For example, methods and materials for detecting the presence or absence of target nucleic acid containing a genetic or epigenetic element, methods and materials for detecting the amount of target nucleic acid containing a genetic or epigenetic element within a sample, kits for detecting the presence or absence of target nucleic acid containing a genetic or epigenetic element, kits for detecting the amount of target nucleic acid containing a genetic or epigenetic element present within a sample, and methods for making such kits are provided.
Claims20
A kit for assessing an organism for a genetic or epigenetic element, wherein said kit comprises:
- (a) a probe nucleic acid coupled to a first restriction endonuclease and comprising a nucleotide sequence complementary to a sequence of a target nucleic acid containing said genetic or epigenetic element, wherein at least a portion of said target nucleic acid is capable of hybridizing to at least a portion of said probe nucleic acid to form a double-stranded portion of nucleic acid comprising a restriction endonuclease cut site,
- (b) a signal expansion nucleic acid (i) coupled to a second restriction endonuclease and (ii) comprising a double-stranded portion of nucleic acid comprising a cut site of said first restriction endonuclease of said probe nucleic acid, and
- (c) a reporter nucleic acid (i) coupled to a label and (ii) comprising a double-stranded portion of nucleic acid comprising a cut site of said first restriction endonuclease of said probe nucleic acid or a cut site of said second restriction endonuclease of said signal expansion nucleic acid.
Dependencies are inferred from the claim text.