Primer set for preparation of NGS library and method and kit for making NGS library using the same
AssigneeRepublic of Korea (National Forensic Service Director Ministry of Public Administration and Security)
InventorsYang-Han Lee, Chong Min Chung, Min-Hee Kim, Eu-Ree An, Min Young Lee
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to a primer set for preparation of a library used for next generation sequencing (NGS), and a method and a kit for making an NGS library using the same. The method for making the next generation sequencing (NGS) library using the primer set according to the present disclosure is able to simply analyze large amounts of samples as compared to the existing method for making a library, which is effectively usable for analysis of target DNA sequences or for database construction, in large amounts of samples.
Claims15
A primer set for preparation of a next generation sequencing (NGS) library comprising:
- a first primer consisting of a first adapter sequence, a first index sequence, a first sequencing sequence, and a first target sequence, and a second primer consisting of a second adapter sequence, a second index sequence, a second sequencing sequence, and a second target sequence, wherein the first primer is selected from the group consisting of SEQ ID NO:
- 27 to SEQ ID NO:
- 34, and the second primer is selected form the group consisting of SEQ ID NO:
- 35 to SEQ ID NO:
- 46.
Dependencies are inferred from the claim text.