Method for reducing dephasing during cluster DNA sequencing
OwnerThomas Frederick Bishopinventor
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Abstract
A method for nucleic acid sequencing, comprising: exposing a plurality of clusters of a plurality of template polynucleotide strands to a series of flows of a plurality of nucleotide or oligonucleotide solutions, wherein the nucleotide solutions or oligonucleotide solutions are flowed in an order of flow which is not a continuous repeat of an ordering of a single flow of each of the nucleotide solutions or oligonucleotide solutions; and obtaining a fluorescence signal indicative of which, if any, nucleotide species or oligonucleotide species incorporated to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands. This method enables dephased amplicons to return in-phase.
Claims28
A method for nucleic acid sequencing, comprising:
- exposing a plurality of clusters of a plurality of template polynucleotide strands with a sequencing primer and a polymerase enzyme operably bound therewith to a series of flows of a plurality of nucleotide solutions,
- wherein the nucleotide solutions are flowed in an order of flow which is not a continuous repeat of an ordering of a single flow of each of the nucleotide solutions;
- and
- obtaining a fluorescence signal indicative of which, if any, nucleotide species incorporated to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands.
A method for nucleic acid sequencing, comprising:
- exposing a plurality of clusters of a plurality of template polynucleotide strands with a sequencing primer operably bound therewith to a series of flows of a plurality of oligonucleotide solutions,
- wherein the oligonucleotide solutions are flowed in an order of flow which is not a continuous repeat of an ordering of a single flow of each of the oligonucleotide solutions;
- and
- obtaining a fluorescence signal indicative of which, if any, oligonucleotide species incorporated to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands.
Dependencies are inferred from the claim text.