Back to results
US 10,329,614 B2Granted
DNA sequencing and epigenome analysis
AssigneeStc.Unm
InventorJeremy Scott Edwards
Life of this patent
Estimating the term…
Abstract
This disclosure describes, in one aspect, methods for DNA sequencing and performing epigenomic analyses. Generally, the methods include immobilizing a plurality of copies of a DNA molecule on a surface, stretching at least a portion of the immobilized DNA molecules, and sequencing at least a portion of the immobilized, stretched DNA molecules.
Claims18
A method comprising
- immobilizing a plurality of copies of a DNA molecule on a surface;
- stretching at least a portion of the immobilized DNA molecules;
- denaturing at least a portion of the immobilized, stretched DNA molecules;
- hybridizing a plurality of probes to at least a portion of the denatured sites of the stretched DNA molecules, thereby creating a hybridized DNA molecule comprising a tag or barcode that identifies the probe on the immobilized DNA,
- wherein:
- each probe comprises at least five nucleotides complementary to at least five nucleotides of a strand of the denatured site of the stretched DNA molecule;
- and
- a tag or barcode further identifies a location corresponding to the portion of the denatured site of the stretched DNA molecule to which the probe carrying the tag is hybridized;
- synthesizing DNA from the probes, wherein the synthesized DNA is complementary to the strand of the stretched DNA to which the probes are hybridized;
- and
- sequencing the DNA synthesized from at least a portion of the probes.
8 more elements
A method comprising
- immobilizing a plurality of copies of a DNA molecule on a surface;
- stretching at least a portion of the immobilized DNA molecules;
- denaturing at least a portion of the immobilized, stretched DNA molecules;
- hybridizing a plurality of probes to at least a portion of the denatured sites of the stretched DNA molecules, wherein each probe comprises at least five nucleotides complementary to at least five nucleotides of a strand of the denatured site of the stretched DNA molecule;
- and
- sequencing at least a portion of the immobilized, stretched DNA molecules, wherein sequencing at least a portion of the immobilized, stretched DNA molecules comprises:
- synthesizing DNA from the probes, wherein the synthesized DNA is complementary to the strand of the stretched DNA to which the probes are hybridized, thereby creating a plurality of elongated probes;
- collecting at least a portion of the elongated probes;
- and
- sequencing the at least a portion of the collected elongated probes.
7 more elements
Dependencies are inferred from the claim text.