Method for detection of Pseudomonas aeruginosa (PA) using volatile biomarkers

A method of determining the presence of Pseudomonas, comprising:

• establishing a gaseous headspace over a surface suspected of containing at least one Pseudomonas strain, the surf ace comprises the internal surface of lungs of a patient and the gaseous head space comprises the gas contained in the lungs before exhaling;
• providing at least one capillary microextraction of volatiles (CMV) sampling device;
• contacting at least a portion of the gaseous headspace with the CMV sampling device, by exhaling at least a portion of the gaseous head space through the CMV sampling device, and wherein at least one component of the gaseous headspace is absorbed by the CMV sampling device;
• desorbing the at least one component of the headspace from the CMV sampling device into an injection port of an analytical device configured to separate, detect, and identify the at least one component of the head space;
• and
• determining from the detected and identified at least one component of the headspace the absence or presence of at least one biomarker comprising undecene of at least one Pseudomonas strain.