Ribosomal protein L40 (RPL40) nucleic acid molecules that confer resistance to coleopteran and hemipteran pests
AssigneeDow AgroSciences LLC
InventorsKenneth E. Narva, Elane Fishilevich, Murugesan Rangasamy, Meghan L. Frey, Wendy Lo, Sarah E. Worden, Premchand Gandra
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Abstract
This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran and/or hemipteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran and/or hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran and/or hemipteran pests, and the plant cells and plants obtained thereby.
Claims35
An isolated nucleic acid comprising a polynucleotide operably linked to a heterologous promoter, wherein the polynucleotide comprises at least a first nucleotide sequence that is selected from the group consisting of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:12;
- the complement of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:12;
- a fragment of at least 35 contiguous nucleotides of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:12;
- the complement of a fragment of at least 35 contiguous nucleotides of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:12;
- a native coding sequence of a Diabrotica organism comprising SEQ ID NO:13;
- the complement of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:13;
- a fragment of at least 35 contiguous nucleotides of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:13;
- the complement of a fragment of at least 35 contiguous nucleotides of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:13;
- a native coding sequence of a Diabrotica organism comprising SEQ ID NO:14;
- the complement of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:14;
- a fragment of at least 35 contiguous nucleotides of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:14;
- and the complement of a fragment of at least 35 contiguous nucleotides of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:14;
- a second nucleotide sequence;
- and,
- a third nucleotide sequence that is the reverse complement of the first nucleotide sequence, wherein the third nucleotide sequence is linked to the first nucleotide sequence by the second nucleotide sequence.
Dependencies are inferred from the claim text.