Fungal resistant plants expressing RLK1
AssigneeBASF Plant Science GmbH
InventorsHolger Schultheiss, Tobias Mentzel
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Abstract
The present invention relates to a method of increasing resistance against fungal pathogens of the order Pucciniales, preferably the family Phacopsoraceae, in plants and/or plant cells. This is achieved by increasing the expression of an RLK1 protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the order Pucciniales, preferably the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an RLK1 protein.
Claims21
A method for preventing, reducing, or delaying Phakopsora infection in a soybean plant, a soybean plant part, or a soybean plant cell, said method comprising:
- providing a transgenic soybean plant, transgenic soybean plant part, or transgenic soybean plant cell with an exogenous nucleic acid encoding a receptor-like kinase 1 (RLK1) protein with at least 72% identity to SEQ ID NO:2,
- wherein the RLK1 protein confers increased resistance against Phakopsora thereto in comparison to a wild type soybean plant, wild type soybean plant part or wild type soybean plant cell,
- wherein the exogenous nucleic acid is in functional linkage with a heterologous promoter;
- and
- growing the transgenic soybean plant, transgenic soybean plant part, or transgenic soybean plant cell in the presence of a fungal pathogen of the genus Phakopsora, wherein Phakopsora infection is prevented, reduced, or delayed in the transgenic soybean plant, transgenic soybean plant part, or transgenic soybean plant cell as compared to a wild type soybean plant, wild type soybean plant part, or wild type soybean plant cell.
A recombinant vector construct comprising in operable linkage:
- (a) a nucleic acid encoding an RLK1 protein comprising an amino acid sequence having at least 72% identity with SEQ ID NO:
- 2,
- (b) a heterologous, fungal-inducible promoter and
- (c) a transcription termination sequence,
- wherein expression of said recombinant vector construct in a plant, plant part, or plant cell confers increased resistance against Phakopsora thereto in comparison to a wild type plant, wild type plant part, or wild type plant cell.
A transgenic plant, transgenic plant part, or transgenic plant cell comprising a nucleic acid encoding an RLK1 protein comprising an amino acid sequence having at least 72% identity with SEQ ID NO:
- 2 operably linked to a heterologous, fungal-inducible promoter.
A transgenic plant, transgenic plant part, or transgenic plant cell comprising a nucleic acid encoding an RLK1 protein comprising an amino acid sequence having at least 90% identity with SEQ ID NO:
- 2 operably linked to a heterologous promoter, wherein the RLK1 protein consists of 650-664 amino acids.
A recombinant vector construct comprising in operable linkage:
- (a) a nucleic acid encoding an RLK1 protein comprising an amino acid sequence having at least 90% identity with SEQ ID NO:
- 2, wherein the RLK1 protein consists of 650-664 amino acids,
- (b) a heterologous promoter and
- (c) a transcription termination sequence,
- wherein expression of said recombinant vector construct in a plant, plant part, or plant cell confers increased resistance against Phakopsora thereto in comparison to a wild type plant, wild type plant part, or wild type plant cell.
Dependencies are inferred from the claim text.