Method to develop high oleic acid soybeans using conventional soybean breeding techniques
AssigneesThe Curators of the University of Missouri, The United States of America as represented by the Secretary of Agriculture
InventorsKristin Bilyeu, Grover Shannon, Jeong-Dong Lee, Anh Tung Pham
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Abstract
The present invention is directed to a soybean plant with mutations in FAD2-1A and FAD2-1B. Moreover, the present invention is directed to seeds from said plants with altered ratios of monosaturated and polyunsaturated fats. In particular, the present invention is directed to plants where the plants exhibit elevated levels of oleic acid.
Claims15
A stably reproducing population of soybean seeds comprising a first polynucleotide sequence encoding a mutant FAD2-1A and a second polynucleotide sequence encoding a mutant FAD2-1B, wherein
- said first polynucleotide sequence is selected from the group consisting of (a) a polynucleotide sequence encoding a FAD2-1A mutant which includes at least one mutation comprising a non-conserved amino acid substitution of serine to asparagine at amino acid position 117 of SEQ ID NO:
- 10, and (b) a polynucleotide sequence encoding M23 mutant characterized by deletion of a FAD2-1A gene having the sequence as set forth in SEQ ID NO:
- 5, said FAD2-1A mutant is nonfunctional or has a reduced activity compared to wild-type FAD2-1A;
- and
- said second nucleotide sequence is selected from the group consisting of (a) a polynucleotide sequence encoding a FAD2-1B mutant which includes at least one mutation comprising a non-conserved amino acid substitution of proline to a polar amino acid selected from the group consisting of arginine, glycine, serine, threonine, cysteine, asparagine, tyrosine, glutamine, lysine and histidine at amino acid position 137 of SEQ ID NO:
- 12, and (b) a polynucleotide sequence encoding a FAD2-1B mutant which includes at least one mutation comprising a non-conserved amino acid substitution of isoleucine to a polar amino acid selected from the group consisting of arginine, glycine, serine, threonine, cysteine, asparagine, tyrosine, glutamine, lysine and histidine at amino acid position 143 of SEQ ID NO:
- 12, said FAD2-1B mutant is nonfunctional or has reduced activity compared to wild-type FAD2-1B,
- wherein oil from said population of soybean seeds has about 65% to about 85% oleic acid content.
A method of producing a soybean plant with seed having an oleic acid content of between about 65% to about 85%, said method comprising:
- (1) crossing a first soybean plant comprising a first polynucleotide sequence selected from the group consisting of (a) a polynucleotide sequence encoding a FAD2-1A mutant which includes at least one mutation comprising a non-conserved amino acid substitution of serine to asparagine at amino acid position 117 of SEQ ID NO:
- 10, and (b) a polynucleotide sequence encoding M23 mutant characterized by deletion of a FAD2-1A gene having the sequence as set forth in SEQ ID NO:
- 5, wherein said FAD2-1A mutant is nonfunctional or has a reduced activity compared to wild-type FAD2-1A, with a second soybean plant comprising a second nucleotide sequence selected from the group consisting of (a) a polynucleotide sequence encoding a FAD2-1B mutant which includes at least one mutation comprising a non-conserved amino acid substitution of proline to a polar amino acid selected from the group consisting of arginine, glycine, serine, threonine, cysteine, asparagine, tyrosine, glutamine, lysine and histidine at amino acid position 137 of SEQ ID NO:
- 12, and (b) a polynucleotide sequence encoding a FAD2-1B mutant which includes at least one mutation comprising a non-conserved amino acid substitution of isoleucine to a polar amino acid selected from the group consisting of arginine, glycine, serine, threonine, cysteine, asparagine, tyrosine, glutamine, lysine and histidine at amino acid position 143 of SEQ ID NO:
- 12, wherein said FAD2-1B mutant is nonfunctional or has reduced activity compared to wild-type FAD2-1B;
- and
- (2) selecting for a progeny soybean plant that comprises said first and second polynucleotide sequences, thereby producing a soybean plant with seed having an oleic acid content of between about 65% to about 85%.
Dependencies are inferred from the claim text.