Agrobacterium rhizogenes transformation and expression of rol genes in kalanchoe
AssigneesUniversity of Copenhagen, Knud Jepsen A/S
InventorsEllen Margrethe Skovsgaard Christensen, Renate Petra Brigitte Müller, Henrik Vlk Lütken, Josefine Nymark Hegelund, Line Jensen, Brian Christensen, Else Bollerup Jensen, Kai Lønne Nielsen
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Abstract
The present disclosure embraces Kalanchoë interspecific hybrid plants, and considers rol transformation in Kalanchoë species and hybrids. Disclosed herein are methodology and the like for producing rol-transformed Kalanchoë interspecific hybrid plants, as well as resultant rol-transformed Kalanchoë interspecific hybrid plants with novel phenotypes.
Claims11
A method for reducing the height of a Kalanchoë interspecific hybrid plant by about 5% to 60%, compared to a wild-type control plant, comprising:
- (a) co-cultivating wild-type Agrobacterium rhizogenes with a Kalanchoë interspecific hybrid plant, wherein said Agrobacterium rhizogenes transfers one or more rol genes into said plant, wherein said one or more rol genes are selected from the group consisting of rolA, rolB, rolC, and rolD, wherein said one or more rol genes include at least rolB, and wherein said one or more rol genes have a nucleic acid sequence selected from the group consisting of SEQ ID Nos:
- 3, 4, 5 and 6, respectively and variants of SEQ ID NOs:
- 3, 4, 5, and 6 that encode a polypeptide selected from the group consisting of SEQ ID NOs:
- 19, 20, 21, and 22, respectively;
- (b) selecting a putatively transformed root of said plant that comprises said one or more rol genes, which root has a hairy root phenotype;
- (c) growing said root on a regeneration medium;
- (d) regenerating a shoot from said root, thereby generating a plantlet,
- (e) growing said plantlet into a mature plant, and
- (f) selecting a plant having reduced height compared to a non-transformed control plant.
A method for reducing the height of a Kalanchoë interspecific hybrid plant by about 5% to about 60%, compared to a wild-type control plant, comprising:
- (a) transforming interspecific hybrid Kalanchoë plant tissue with Agrobacterium rhizogenes, wherein said Agrobacterium rhizogenes delivers and integrates one or more rol genes into hybrid plant genome, wherein said one or more rol genes are selected from the group consisting of rolA, rolB, rolC, and rolD, wherein said one or more rol genes include at least rolB, and wherein said one or more rol genes have a nucleic acid sequence selected from the group consisting of SEQ ID Nos:
- 3, 4, 5 and 6, respectively and variants of SEQ ID NOs:
- 3, 4, 5, and 6 that encode a polypeptide selected from the group consisting of SEQ ID NOs:
- 19, 20, 21, and 22, respectively;
- (b) selecting a putatively transformed root having a hairy root phenotype;
- (c) growing said root on a regeneration medium;
- (d) regenerating a shoot from said root, thereby generating a plantlet;
- (e) growing said plantlet into a mature plant, and;
- (f) selecting a plant having a reduced height by about 5% to about 60% compared to the height of a non-transformed control plant of the same species.
A rol-transformed Kalanchoë interspecific hybrid with intermediate height, wherein said intermediate height is a reduction of height by about 5% to about 60% of the height of a control, non-transformed Kalanchoë interspecific hybrid plant, wherein the rol-transformed Kalanchoë interspecific hybrid has been transformed with one or more rol genes, wherein said one or more rol genes are selected from the group consisting of rolA, rolB, rolC, and rolD, wherein said one or more rol genes include at least rolB, and wherein said one or more rol genes have a nucleic acid sequence selected from the group consisting of SEQ ID Nos:
- 3, 4, 5 and 6, respectively and variants of SEQ ID NOs:
- 3, 4, 5, and 6 that encode a polypeptide selected from the group consisting of SEQ ID NOs:
- 19, 20, 21, and 22, respectively.
Dependencies are inferred from the claim text.