Composition for reducing cellular senescence level including activity inhibitor inhibiting DCUN1D3 activity or expression inhibitor inhibiting expression of DCUN1D3-encoding gene and use thereof
AssigneeSamsung Electronics Co., Ltd.
InventorsMyoungsoon Kim, Yongsub Kim, Young-Sam Lee
Life of this patent
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Abstract
Provided is a composition and method for reducing a cellular senescence level by inhibiting the activity or expression of one or more of DCUN1D3 protein or gene encoding same and a polynucleotide having a nucleotide sequence of SEQ ID NO: 5, as well as a method of treating a disease or a disease symptom associated with an increased cellular senescence level in a mammal.
Claims7
A method of reducing cellular senescence in a mammal in need thereof, the method comprising administering to the mammal a non-natural composition comprising an activity inhibitor inhibiting activity of one or more of DCN1, defective in cullin neddylation 1, domain containing 3 (DCUN1D3) protein and a polynucleotide having a nucleotide sequence of SEQ ID NO:
- 5, or an expression inhibitor that inhibits expression of one or more of a gene encoding DCUN1D3 and a gene encoding the nucleotide sequence of SEQ ID NO:
- 5,
- wherein the composition reduces markers of cellular senescence when administered to a cell;
- the expression inhibitor is a vector encoding a small hairpin RNA (shRNA) comprising a nucleotide sequence of SEQ ID NO:
- 1 and a nucleotide sequence of SEQ ID NO:
- 2;
- and the activity inhibitor is small interference RNA (siRNA) against a polynucleotide having a nucleotide sequence of SEQ ID NO:
- 5, comprising polynucleotides of SEQ ID NO:
- 10 and 11, SEQ ID NO:12 and 13, or SEQ ID NO:
- 14 and 15.
A method of treating a disease or a disease symptom associated with increased cellular senescence in a mammal with said disease or disease symptom, the method comprising administering to the mammal a non-natural composition comprising an activity inhibitor inhibiting activity of one or more of DCN1, defective in cullin neddylation 1, domain containing 3 (DCUN1D3) protein and a polynucleotide having a nucleotide sequence of SEQ ID NO:
- 5, or an expression inhibitor inhibiting expression of one or more of a gene encoding DCUN1D3 and a gene encoding the nucleotide sequence of SEQ ID NO:
- 5,
- wherein the composition reduces markers of cellular senescence when administered to a cell;
- the expression inhibitor is a vector encoding a small hairpin RNA (shRNA) comprising a nucleotide sequence of SEQ ID NO:
- 1 and a nucleotide sequence of SEQ ID NO:
- 2;
- and the activity inhibitor is small interference RNA (siRNA) against a polynucleotide having a nucleotide sequence of SEQ ID NO:
- 5, comprising polynucleotides of SEQ ID NO:
- 10 and 11, SEQ ID NO:12 and 13, or SEQ ID NO:
- 14 and 15.
A method of reducing a lipofuscin level in a mammalian cell, the method comprising administering to a mammal in need thereof a non-natural composition comprising an activity inhibitor inhibiting activity of one or more of DCN1, defective in cullin neddylation 1, domain containing 3 (DCUN1D3) protein and a polynucleotide having a nucleotide sequence of SEQ ID NO:
- 5, or an expression inhibitor inhibiting expression of one or more of a gene encoding DCUN1D3 and a gene encoding the nucleotide sequence of SEQ ID NO:
- 5,
- wherein the composition reduces markers of cellular senescence when administered to a cell;
- the expression inhibitor is a vector encoding a small hairpin RNA (shRNA) comprising a nucleotide sequence of SEQ ID NO:
- 1 and a nucleotide sequence of SEQ ID NO:
- 2;
- and the activity inhibitor is small interference RNA (siRNA) against a polynucleotide having a nucleotide sequence of SEQ ID NO:
- 5, comprising polynucleotides of SEQ ID NO:
- 10 and 11, SEQ ID NO:12 and 13, or SEQ ID NO:
- 14 and 15.
Dependencies are inferred from the claim text.