Interfering RNA molecules
AssigneeSilence Therapeutics GmbH
InventorsKlaus Giese, Jörg Kaufmann, Anke Klippel-Giese
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Abstract
The present invention is related to a ribonucleic acid comprising a double stranded structure whereby the double-stranded structure comprises a first strand and a second strand, whereby the first strand comprises a first stretch of contiguous nucleotides and whereby said first stretch is at least partially complementary to a target nucleic acid, and the second strand comprises a second stretch of contiguous nucleotides whereby said second stretch is at least partially identical to a target nucleic acid, and whereby the double stranded structure is blunt ended.
Claims24
A double stranded siRNA molecule against a target nucleic acid, wherein the double stranded siRNA molecule comprises a first strand and a second strand, wherein the first strand comprises a first stretch that is complementary to the target nucleic acid, wherein the second strand comprises a second stretch that has the same length as the first stretch and is complementary to the first stretch, wherein the first strand and the second strand form a double-stranded structure comprising the first stretch and the second stretch, wherein the first stretch and the second stretch each has the length of 18-19 nucleotides,
- wherein the first stretch and the second stretch each comprises a plurality of groups of one or more 2′-O-methylribonucleotides, wherein within each stretch each group of the 2′-O-methyl ribonucleotide is flanked on one or both ends by a flanking group of one or more unmodified ribonucleotides,
- wherein each 2′-O-methyl ribonucleotide on the first stretch corresponds to an unmodified ribonucleotide on the second stretch, and
- wherein each 2′-O-methyl ribonucleotide on the second stretch corresponds to an unmodified ribonucleotide on the first stretch.
Dependencies are inferred from the claim text.