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US 10,329,543 B2Granted
Modified stem cell memory T cells, methods of making and methods of using same
AssigneePoseida Therapeutics, Inc.
InventorsEric Ostertag, Devon Shedlock
Life of this patent
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Abstract
The disclosure provides a method of producing modified stem memory T cells (e.g. CAR-T cells) for administration to a subject as, for example an adoptive cell therapy.
Claims28
A method of producing a plurality of expanded modified T cells, comprising
- introducing into a plurality of primary human T cells (a) a transposon composition comprising a transposon comprising an antigen receptor, a therapeutic protein or a sequence encoding the same and (b) a transposase composition comprising a transposase or a sequence encoding the transposase to produce a plurality of modified T cells, under conditions that preserve desirable stem-like properties of the plurality of modified T cells;
- wherein the conditions comprise culturing the plurality of modified T cells in vitro in a liquid culture media comprising a sterol;
- an alkane;
- phosphorus and one or more of an octanoic acid, a palmitic acid, a linoleic acid, and an oleic acid;
- wherein at least 25% of the plurality of modified T cells expresses one or more cell-surface marker(s) of a stem memory T cell (TSCM) or a TSCM-like cell and wherein the one or more cell-surface marker(s) comprises CD62L and CD45RA.
2 more elements
Dependencies are inferred from the claim text.