System and method for cold atmospheric plasma treatment on cancer stem cells

A method for treating cancer stem cells with cold atmospheric plasma using a cold atmospheric plasma system for producing a cold atmospheric plasma jet, said cold atmospheric plasma system having a source of high voltage power, a source of an inert gas, and a cold plasma delivery apparatus having a housing, a channel within said housing connected to said source of insert gas and having an exit port through which said inert gas exits said housing, and a central electrode within said housing, the method comprising the steps of:

• applying a cold plasma jet from said cold atmospheric plasma system onto target cancer stem cells on at least two different days in a period of four consecutive days, wherein each application of cold atmospheric plasma to said target cancer stem cells comprises the steps of:
• placing said exit port 5 cm or less from target cancer stem cells;
• flowing said inert gas from said source through said housing at a flow rate of 5-10 ml/minute;
• applying a voltage of 2-5 kV at a frequency of 20-35 kHz to said central electrode to produce a cold plasma jet from said exit port;
• directing said cold plasma jet onto the target cancer stem cells;
• applying said cold plasma jet onto said target cancer stem cells for at least 2 minutes.

A method for treating cancer stem cells with cold atmospheric plasma using a cold atmospheric plasma system having a source of high voltage power, a source of an inert gas, and a cold plasma delivery apparatus having a housing, a channel within said housing connected to said source of insert gas and having an exit port through which said inert gas exits said housing, and a central electrode within said housing, the method comprising the steps of:

• placing said exit port 5 cm or less from target cancer stem cells;
• flowing said inert gas from said source through said housing at a flow rate of 5-10 ml/minute;
• applying a voltage of 2-5 kV at a frequency of 20-35 kHz to said central electrode to produce a cold plasma jet from said exit port;
• directing said cold plasma jet onto the target cancer stem cells;
• and
• applying said cold plasma jet onto said target cancer stem cells for at least 2 minutes.