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US 10,329,528 B2Granted
Method of forming microparticles for use in cell seeding
AssigneeThe Curators of the University of Missouri
InventorsSutapa Barua, Chase Herman
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Abstract
The present invention is directed to methods for forming microparticles useful for cell seeding and for conjugating protein to the surface of the microparticles. The method comprises co-injecting an organic solution of PLGA or other polymer with an aqueous solution into a flow focusing tube.
Claims65
A method for forming microparticles useful for cell seeding comprising:
- obtaining an organic solution comprising poly(D,L-lactide-co-glycolide) (PLGA);
- obtaining a first aqueous solution comprising polyvinyl alcohol (PVA);
- obtaining a second aqueous solution comprising PVA in a higher concentration than said first aqueous solution;
- co-injecting a stream of the organic solution and a carrier stream of the first aqueous solution into a flow focusing tube to form organic droplets within the carrier stream;
- introducing the carrier stream comprising the organic droplets into the second aqueous solution to form microparticles of the PLGA in the second aqueous solution;
- evaporating the organic solvent;
- and
- removing the PVA, wherein the ratio of the PLGA to the organic solvent is from 5% (w/v) to 15% (w/v).
5 more elements
A method for forming microparticles useful for cell seeding comprising:
- obtaining an organic solution comprising poly(D,L-lactide-co-glycolide) (PLGA) and polyethylene glycol (PEG);
- obtaining a first aqueous solution comprising polyvinyl alcohol (PVA);
- obtaining a second aqueous solution comprising PVA in a higher concentration than said first aqueous solution;
- co-injecting a stream of the organic solution and a carrier stream of the first aqueous solution into a flow focusing tube to form organic droplets within the carrier stream;
- introducing the carrier stream comprising the organic droplets into the second aqueous solution to form microparticles of the PLGA in the second aqueous solution;
- evaporating the organic solvent;
- and
- removing the PVA, wherein the ratio of PLGA to PEG is greater than 70:1.
5 more elements
A method for forming microparticles useful for cell seeding comprising:
- obtaining an organic solution comprising poly(D,L-lactide-co-glycolide) (PLGA);
- obtaining a first aqueous solution comprising polyvinyl alcohol (PVA);
- obtaining a second aqueous solution comprising PVA in a higher concentration than said first aqueous solution;
- co-injecting a stream of the organic solution and a carrier stream of the first aqueous solution into a flow focusing tube to form organic droplets within the carrier stream;
- introducing the carrier stream comprising the organic droplets into the second aqueous solution to form microparticles of the PLGA in the second aqueous solution;
- evaporating the organic solvent;
- and
- removing the PVA, wherein the ratio of the organic solution to the first aqueous solution is 1:1 to 1:10.
5 more elements
A method for forming microparticles useful for cell seeding comprising:
- obtaining an organic solution comprising poly(D,L-lactide-co-glycolide) (PLGA);
- obtaining a first aqueous solution comprising polyvinyl alcohol (PVA);
- obtaining a second aqueous solution comprising PVA in a higher concentration than said first aqueous solution;
- co-injecting a stream of the organic solution and a carrier stream of the first aqueous solution into a flow focusing tube to form organic droplets within the carrier stream;
- introducing the carrier stream comprising the organic droplets into the second aqueous solution to form microparticles of the PLGA in the second aqueous solution;
- evaporating the organic solvent;
- removing the PVA, and after said removing step, the step of stretching the PLGA microparticles into microdisks and microrods.
4 more elements
Dependencies are inferred from the claim text.