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US 10,329,527 B2Granted
Spheroid forming culture container using temperature-sensitive glycol chitosan derivative, and spheroid forming method using same
AssigneesThe Industry & Academic Coopertaion In Chungnam National University (Iac), Korea Research Institute of Chemical Technology
InventorsKang Moo Huh, Sun Woong Kang, Han Chang Kang, Hye Eun Shim, Ik Sung Cho, Myeong Ok Cho
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Abstract
Disclosed is a spheroid forming culture container using a temperature-sensitive glycol chitosan derivative and a spheroid forming method using the same. In the disclosed spheroid forming culture container, a surface of a culturing space is coated with a glycol chitosan derivative having reversible sol-gel transition characteristic depending on temperature.
Claims7
A spheroid forming method comprising:
- using a culture container in which a surface of a culturing space is coated with a glycol chitosan derivative of the following Chemical Formula 1:
- wherein,
- R1 is a C1-C18 alkyl group;
- X, y, and z are an integer from 10 to 10000;
- and mol % thereof is 0.05≤x≤0.8, 0.05≤y≤0.15, and 0.05≤z≤0.9;
- placing cells for culturing in the culture container;
- and
- culturing the cells in the culture container to form spheroids.
5 more elements
Dependencies are inferred from the claim text.