Process for immobilizing one or more receptor biomolecules on one or more solid surfaces

An asymmetric immobilization process that immobilizes a plurality of receptor biomolecules on a plurality of micro-cantilevers comprising:

• activating the plurality of micro-cantilevers using an oxidizing agent;
• treating the plurality of micro-cantilevers with aminosilane to obtain a plurality of amine functionalized micro-cantilevers;
• washing the plurality or amine functionalized micro-cantilevers using organic solvents;
• treating the plurality of receptor biomolecules with a 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) to obtain a plurality of site-specific EDC activated receptor biomolecules;
• immobilizing the plurality of site-specific EDC activated receptor biomolecules on a first layer of the plurality of amine functionalized micro-cantilevers, wherein the immobilizing comprises treating the plurality of site-specific EDC activated receptor biomolecules with amine groups of the first layer of the plurality of amine functionalized micro-cantilevers to form a covalent amide bond, wherein the EDC is hydrolyzed from the plurality of site-specific EDC activated receptor biomolecules when the plurality of site-specific EDC activated receptor biomolecules binds with amine groups of the plurality of amine functionalized micro-cantilevers;
• coating a second layer of the plurality of amine functionalized micro-cantilevers with least one of (a) acyl chloride, and (b) anhydrides;
• washing the plurality of site-specific EDC activated receptor biomolecules that are excess from the plurality of amine functionalized micro-cantilevers using at least one of (a) a phosphate buffer saline, (b) de-ionised water;
• and
• treating the first layer of the plurality of amine functionalized micro-cantilevers with bovine serum albumin to block unbound amine groups of the plurality of amine functionalized micro-cantilevers.