Specific detection tool for mesenchymal and epithelial-mesenchymal transformed circulating tumor cells
AssigneeBoard of Regents, The University of Texas System
InventorsArun Satelli, Shulin Li
Life of this patent
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Abstract
The present invention includes the discovery of cell-surface vimentin as a novel biomarker to detect and isolate mesenchymal and epithelial-mesenchymal transformed circulating tumor cells from blood of cancer patients. In addition, an antibody specific for the detection of cell-surface vimentin on circulating tumor cells and the use the antibody to detect, enumerate, and isolate CTC are provided.
Claims16
An isolated antibody-cell complex comprising an isolated and captured complex of a monoclonal antibody or an antigen binding fragment thereof bound to vimentin on the cell surface of a non-permeabilized metastatic tumor cell, wherein the metastatic tumor cell is a circulating metastatic tumor cell of a solid tumor, wherein the antibody or antigen binding fragment comprises:
- (a) a first VH CDR identical to SEQ ID NO:
- 3;
- (b) a second VH CDR identical to SEQ ID NO:
- 4;
- (c) a third VH CDR identical to SEQ ID NO:
- 5;
- (d) a first VL CDR identical to SEQ ID NO:
- 6;
- (e) a second VL CDR identical to SEQ ID NO:
- 7;
- and
- (f) a third VL CDR identical to SEQ ID NO:
- 8.
Dependencies are inferred from the claim text.