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US 10,329,322 B2Granted
Methods for reducing aggregate content of protein preparations by treatment with aryl anions
AssigneeAgency for Science, Technology and Research
InventorPeter Stanley Gagnon
Life of this patent
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Abstract
A method of reducing the aggregate content in a preparation having a target protein includes contacting the preparation with an aryl anion to form a mixture and contacting the mixture with at least one electropositive solid to remove excess aryl anion.
Claims12
A method of reducing the aggregate content in a preparation comprising a target protein, the method comprising:
- (a) contacting the preparation with methyl blue to form a mixture;
- (b) contacting the mixture with at least one electropositive solid to remove excess methyl blue;
- and
- (c) separating the target protein from the electropositive solid.
1 more element
A method of reducing the aggregate content in a preparation comprising a soluble target protein, the method comprising:
- (a) contacting the preparation with methyl blue to form a mixture, wherein the methyl blue is present at a concentration from about 0.01% to about 0.1%;
- (b) contacting the mixture with an electropositive solid;
- and
- (c) separating the soluble target protein from the electropositive solid.
1 more element
Dependencies are inferred from the claim text.