Live-attenuated vaccine against plague
AssigneeBoard of Regents, The University of Texas System
InventorsAshok K. Chopra, Jian Sha
Life of this patent
Estimating the term…
Abstract
Provided herein is a genetically modified Y. pestis that includes three alterations compared to a control Y. pestis. Two alterations include decreased mRNA, decreased protein, or a combination thereof, encoded by a lpp coding region and encoded by a msbB coding region. The third alteration is selected from an alteration of an intergenic region between the coding regions ypo1119 and ypo1120, and decreased mRNA, decreased protein, or a combination thereof, encoded by a coding region selected from pla, ypol717, ypmt1.80c, rbsA (ypo2500), ypo0498, vasK (ypo3603), ypo3164, hxuB (ypo3248), ypo1616, ypo1119, ypo1120, and ail. Also provided are compositions that include the genetically modified Y. pestis, and methods of using the genetically modified Y. pestis.
Claims19
A genetically modified Y. pestis, comprising three alterations compared to a control Y. pestis,
- wherein the first alteration comprises a mutation in a first coding region encoding a first protein having at least 80% identity to the amino acid sequence of SEQ ID NO:49, wherein the first protein is undetectable in the genetically modified Y. pestis,
- wherein the second alteration comprises a mutation in a second coding region encoding a second protein having at least 80% identity to the amino acid sequence of SEQ ID NO:50, wherein the second protein is undetectable in the genetically modified Y. pestis,
- wherein the third alteration comprises a mutation in either a third coding region or a deletion of an intergenic region, wherein the third coding region encodes a third protein having at least 80% identity to the amino acid sequence of SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, SEQ ID NO:60, SEQ ID NO:61, or SEQ ID NO:62, and wherein the intergenic region has at least 80% identity to SEQ ID NO:2, wherein the third protein is undetectable in the genetically modified Y. pestis, and
- wherein the genetically modified Y. pestis, is attenuated.
A genetically modified Y. pestis comprising three alterations compared to a control Y. pestis,
- wherein the first alteration comprises a mutation in a first coding region encoding a first protein having at least 80% identity to SEQ ID NO:49, wherein the first protein is undetectable in the genetically modified Y. pestis,
- wherein the second alteration comprises a mutation in a second coding region encoding a second protein having at least 80% identity to SEQ ID NO:50, wherein the second protein is undetectable in the genetically modified Y. pestis,
- wherein the third alteration comprises a mutation in a third coding region encoding a third protein having at least 80% identity to the amino acid sequence of SEQ ID NO:62, SEQ ID NO:54, or SEQ ID NO:56, wherein the third protein is undetectable in the genetically modified Y. pestis,
- wherein the genetically modified Y. pestis is attenuated.
Dependencies are inferred from the claim text.