Back to results
US 10,328,133 B2Granted
Continuous cell programming devices
AssigneesPresident and Fellows of Harvard College, Dana-Farber Cancer Institute, Inc.
InventorsDavid J. Mooney, Omar Ali, Glenn Dranoff
Life of this patent
Estimating the term…
Abstract
The present invention comprises compositions, methods and devices for creating an infection-mimicking environment within a polymer scaffold to stimulate antigen-specific dendritic cell activation. Devices of the present invention are used to provide protective immunity to subjects against infection and cancer.
Claims8
A method of continuous in situ dendritic cell programming, comprising administering to a subject a device comprising
- a scaffold polymer selected from the group consisting of poly-lactide-co-glycolide (PLG), alginate, xantham gum, gellan, or emulsan;
- a tumor antigen;
- a recruitment composition comprising a cytokine, which comprises a granulocyte macrophage colony stimulating factor (GM-CSF) that recruits one or more dendritic cells to temporarily reside within said device;
- and
- a positively charged polyethylenimine (PEI)-condensed toll-like receptor-9 (TLR-9)-activating CpG-ODN which is retained in or on said scaffold polymer,
- wherein said device attracts a dendritic cell and enhances uptake of said CpG-ODN into said dendritic cell, thereby continuously stimulating said dendritic cell to induce an immune response, and wherein said PEI condensed CpG-ODN overcomes the trapping effect of the cytokine on said dendritic cell, thereby inducing said dendritic cell to migrate away from said device.
3 more elements
Dependencies are inferred from the claim text.