Methods for the production of a cosmetic composition from hatching fluid and uses thereof for improving the cosmetic appearance of skin
AssigneeAqua Bio Technology Asa
InventorsBernt T H. Walther, Hans Kristian Leren
Life of this patent
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Abstract
The present invention relates to cosmetic compositions obtained or obtainable from Salmonidae hatching fluid, methods of producing said compositions and their use in various cosmetic applications to the skin, particularly for reducing or preventing the cosmetic appearance or prevalence of wrinkles, fine lines, hyperpigmentation, laxity, dry skin, scaling and/or transepidermal water loss in skin of a mammalian animal.
Claims15
A method for reducing the cosmetic appearance or prevalence of wrinkles, fine lines and/or laxity in skin of a mammalian animal, comprising administering topically a cosmetic composition to skin having wrinkles, fine lines and/or laxity of said animal, wherein the administration of said cosmetic composition results in the reduction in the number and/or size of skin wrinkles and/or fine lines and/or a reduction in skin laxity and wherein said cosmetic composition is obtained by a method consisting of the steps of:
- a) suspending Salmonidae eggs in water;
- b) inducing synchronized hatching of said eggs;
- c) filtering the hatched eggs to obtain hatching fluid;
- and
- d) filtering the hatching fluid from step c), wherein the step of filtering the hatching fluid comprises at least the steps of:
- (i) filtering the hatching fluid using a filter with a pore size of at least 5 μm, and collecting the filtrate;
- (ii) filtering the filtrate from step (i) using a filter with a pore size of 0.30-0.60 μm, and collecting the filtrate;
- (iii) exchanging water in the filtrate from step (ii) with a pharmaceutically acceptable buffer by performing diafiltration using a filter with an exclusion size of less than 10 kDa to obtain a filtrate solution;
- (iv) filtering the filtrate solution from step (iii) using a filter with a pore size of 0.15-0.30 μm, and collecting the filtrate;
- (v) optionally repeating the diafiltration step of step (iii);
- (vi) optionally diluting the filtrate solution or the filtrate from step (iii), (iv) or (v);
- and
- (vii) preparing a cosmetic composition from the filtrate solution or the filtrate from step (iv), (v) or (vi), wherein the cosmetic composition comprises 0.1-5% volume of the filtrate solution or the filtrate from step (iv), (v) or (vi) per unit volume of cosmetic composition,
- wherein the filtrate solution or the filtrate from step (iv), (v) or (vi) is substantially free of components derived from the Salmonidae eggs other than polypeptides or portions thereof.
A method of preparing a cosmetic composition consisting of the steps of:
- a) suspending Salmonidae eggs in water;
- b) inducing synchronized hatching of said eggs;
- c) filtering the hatched eggs to obtain hatching fluid;
- and
- d) filtering the hatching fluid to obtain the composition, wherein the step of filtering the hatching fluid comprises at least the steps of:
- (i) filtering the hatching fluid using a filter with a pore size of at least 5 μm, and collecting the filtrate;
- (ii) filtering the filtrate from step (i) using a filter with a pore size of 0.30-0.60 μm, and collecting the filtrate;
- (iii) exchanging water in the filtrate from step (ii) with a pharmaceutically acceptable buffer by performing diafiltration using a filter with an exclusion size of less than 10 kDa to obtain a filtrate solution;
- (iv) filtering the filtrate solution from step (iii) using a filter with a pore size of 0.15-0.30 μm, and collecting the filtrate;
- (v) optionally repeating the diafiltration step of step (iii);
- (vi) optionally diluting the filtrate solution or the filtrate from step (iii), (iv) or (v);
- and
- (vii) preparing said cosmetic composition from the filtrate solution or the filtrate from step (iv), (v) or (vi), wherein the cosmetic composition comprises 0.1-5% volume of the filtrate solution or the filtrate from step (iv), (v) or (vi) per unit volume of cosmetic composition,
- wherein the filtrate solution or the filtrate from step (iv), (v) or (vi) is substantially free of components derived from the Salmonidae eggs other than polypeptides or portions thereof.
Dependencies are inferred from the claim text.