Alpha-amylase variants
AssigneeDsm IP Assets B.V.
InventorsRene Marcel De Jong, Hanna Elsabet Abbas, Evert Tjeerd Van Rij
Life of this patent
Estimating the term…
Abstract
A variant polypeptide having alpha-amylase activity, wherein the variant has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue. Such a variant polypeptide may be used in the preparation of a baked product.
Claims32
A variant polypeptide having alpha-amylase activity, wherein the variant polypeptide has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO:2, comprises at least one substitution of an amino acid residue corresponding to amino acid 200, said position being defined with reference to SEQ ID NO:2, wherein the variant is at least 90% identical to SEQ ID NO:2, and wherein the variant has one or more altered properties as compared with a reference polypeptide having alpha-amylase activity.
A method of producing an alpha-amylase polypeptide variant, comprising:
- a) selecting a polypeptide having alpha-amylase activity;
- b) substituting at least one amino acid residue corresponding to amino acid 200, said position being defined with reference to SEQ ID NO:2;
- c) optionally substituting one or more further amino acids selected from the group consisting of 4, 6, 13, 14, 15, 16, 20, 45, 47, 51, 54, 61, 68, 69, 70, 71, 72, 73, 74, 75, 77, 78, 80, 82, 87, 88, 94, 95, 100, 103, 104, 117, 124, 125, 126, 128, 130, 133, 134, 136, 143, 144, 146, 168, 174, 177, 178, 183, 186, 188, 189, 190, 194, 195, 199, 201, 204, 207, 210, 214, 217, 219, 222, 225, 227, 233, 234, 235, 236, 240, 251, 252, 254, 258, 259, 260, 261, 262, 263, 264, 266, 267, 269, 271, 273, 281, 282, 283, 284, 286, 288, 299, 322, 323, 325, 327, 328, 331, 334, 350, 356, 358, 367, 370, 371, 374, 377, 378, 388, 391, 414, 421, 422, 445, 450, 467, 488, 505, 536, 548, 554, 583, 588, 603, 637, 648, 651, 652, 660, 676, and 677,
- said positions being defined with reference to SEQ ID NO:2;
- wherein the variant is at least 90% identical to SEQ ID NO:
- 2;
- d) preparing the polypeptide variant resulting from steps a)-c);
- e) determining a property of the polypeptide variant;
- and
- f) selecting a polypeptide variant having an altered property in comparison a reference polypeptide having alpha-amylase activity, thereby to produce an alpha-amylase polypeptide variant.
Dependencies are inferred from the claim text.