Modulation of calcium ion homeostasis in harvested transplantable hearts
AssigneeTevosol, Inc.
InventorsDarren Freed, Christopher White, Larry Hryshko
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Abstract
An oxygenated cardioplegic composition for immediate reperfusion of a donor heart after its procurement. The composition comprises an adenosine-lidocaine mixture for causing immediate cessation of the heart's systolic function upon contact; a normokalemic concentration of potassium ions; a concentration of Ca2+ ions selected to maintain the intracellular level of Ca2+ ions in the harvested heart muscle cells at about 10−4 mmol/L; and a pH of 6.9. The oxygenated cardioplegic composition is pre-warmed to about 35° C. and then used for immediate reperfusion of a donor heart for at least three minutes after its procurement.
Claims6
A cardioplegic composition comprising:
- 400 μmol/L of adenosine;
- 50 μmol/L of lidocaine;
- 10.0 mmol/L of glucose;
- 131.8 mmol/L of NaCl;
- 5.9 mmol/L of KCl;
- 14.0 mmol/L of NaHCO3;
- 1.2 mmol/L of NaH2PO4;
- 0.22 mmol/L of CaCl2;
- 13.0 mmol/L of MgCl2;
- 10.0 IU/L of insulin
- 120.0 mmol/L of D-mannitol;
- 1.0 mmol/L of pyruvate;
- and
- 3.0 mmol/L of reduced glutathione,
- wherein the cardioplegic composition has a pH of 6.6 to 6.9.
Dependencies are inferred from the claim text.