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US 10,323,262 B2Granted
Microorganism producing O-phosphoserine and a method for producing O-phosphoserine or L-cysteine using the same
AssigneeCj Cheiljedang Corporation
InventorsSol Kim, In Hwa Yoo, Jin Sook Chang, Hye Won Kim
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Abstract
The present invention relates to a microorganism, wherein the activity of a polypeptide capable of exporting O-phosphoserine (OPS) is enhanced, and a method of producing O-phosphoserine, cysteine, or a cysteine derivative using the microorganism.
Claims14
A microorganism capable of producing O-phosphoserine (OPS), in which an OPS exporting activity of a polypeptide, which has the amino acid sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 2 and is capable of exporting O-phosphoserine, is enhanced compared to its endogenous activity;
- and
- an activity of phosphoserine phosphatase (SerB) is weakened compared to its endogenous activity.
1 more element
A method for producing O-phosphoserine (OPS), comprising:
- culturing a microorganism capable of producing O-phosphoserine, in which an OPS exporting activity of a polypeptide which has the amino acid sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 2 and is capable of exporting O-phosphoserine is enhanced compared to its endogenous activity, in a medium;
- and
- separating O-phosphoserine from the microorganism capable of producing O-phosphoserine, or the medium for the same.
2 more elements
A method for producing cysteine or a derivative thereof, comprising:
- a) producing O-phosphoserine (OPS) by culturing a microorganism capable of producing O-phosphoserine (OPS), in which an OPS exporting activity of a polypeptide, which has the amino acid sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 2 and is capable of exporting O-phosphoserine, is enhanced compared to its endogenous activity in a medium;
- and
- b) reacting the O-phosphoserine (OPS) produced in a) or a culture containing the same with a sulfide, in the presence of O-phosphoserine sulfhydrylase (OPSS) or a microorganism capable of expressing the same.
2 more elements
Dependencies are inferred from the claim text.