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US 10,323,232 B2Granted
Metabolically engineered methanotrophic, phototrophic microorganisms
AssigneeMogene LC
InventorsAbhay Singh, Himadri Pakrasi, Ganesh M. Kishore
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Abstract
This disclosure relates to the engineering of phototrophic microorganisms for conversion of alkanes into higher-value products. Recombinant phototrophic organisms such as cyanobacteria can be engineered, optionally in a modular format, to express enzymes involved in converting methane to methanol, methanol to formaldehyde, formaldehyde to central metabolic pathway intermediates, and such intermediates to n-butanol.
Claims8
A recombinant Synechocystis sp. PCC 6803, comprising:
- (i) a gene encoding a soluble methane monooxygenase (sMMO);
- (ii) a gene encoding a methanol dehydrogenase (MDH);
- (iii) a gene encoding a hexulose-6-phosphate synthase (HPS);
- and
- (iv) a gene encoding a 6-phosphate-3-hexuloisomerase (PHI).
2 more elements
Dependencies are inferred from the claim text.