Device for controlled apical flow in cell culture inserts
AssigneesNortheastern University, Massachusetts Institute of Technology
InventorsTranson V. Nguyen, Collin D. J. Edington, Emily C. Suter, Rebecca Lyn Carrier, David L. Trumper, Linda G. Griffith
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Abstract
A device for controlling apical flow to a cell culture includes an apical insert that defines at least one inlet channel extending from an inlet port to an apical feed port and at least one outlet channel extending from an apical effluent port to an outlet port. The apical insert includes a projecting portion configured to extend into a cell culture insert to a depth that is less than a depth of the cell culture insert, and a contact surface configured to maintain a spatial relationship between the projecting portion and the cell culture insert.
Claims18
A device for controlling apical flow to a cell culture, comprising:
- an apical insert including (i) a projecting portion configured to extend into a cell culture insert to a depth that is less than a depth of the cell culture insert, the projecting portion including an apical surface and at least one peripheral surface, and (ii) a contact surface configured to maintain a spatial relationship between the projecting portion and the cell culture insert, the apical insert defining at least one inlet channel extending from an inlet port to an apical feed port and at least one outlet channel extending from an apical effluent port to an outlet port, the apical feed port and the apical effluent port each being disposed at the at least one peripheral surface of the projecting portion.
A system for controlling apical flow to a cell culture, comprising:
- a cell culture insert;
- an apical insert including a projecting portion configured to extend into a cell culture insert to a depth that is less than a depth of the cell culture insert, the projecting portion including an apical surface and at least one peripheral surface, and a contact surface configured to maintain a spatial relationship between the projecting portion and the cell culture insert, the apical insert defining at least one inlet channel extending from an inlet port to an apical feed port and at least one outlet channel extending from an apical effluent port to an outlet port, the apical feed port and the apical effluent port each being disposed at the at least one peripheral surface of the projecting portion;
- and
- at least one seal configured to seal the insert against the cell culture insert.
Dependencies are inferred from the claim text.