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US 10,316,342 B2Granted
Cell-free production of sugars
AssigneeGreenLight Biosciences, Inc.
InventorsDaniel MacEachran, Drew S. Cunningham, William Jeremy Blake, Matthew Eduardo Moura
Life of this patent
Estimating the term…
Abstract
Provided herein, in some embodiments, are systems, methods, and compositions (e.g., cells and cell lysates) for enzymatically converting a polymeric glucose carbohydrate (e.g., starch) to sugar.
Claims30
A method for producing allulose, the method comprising:
- converting fructose 6-phosphate (F6P) to allulose 6-phosphate (A6P), catalyzed by an allulose 6-phosphate epimerase (A6PE) selected from the group consisting of Thermoanaerobacterium thermosaccharolyticum A6PE, Hydrogenivirga sp. 128-5-R1-1 A6PE, and Brevibacillus thermoruber A6PE;
- and
- converting A6P to allulose, catalyzed by Ruminiclostridium thermocellum allulose 6-phosphate phosphatase (A6PP).
Dependencies are inferred from the claim text.