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US 10,316,102 B2Granted
Car expression vector and car-expressing T cells
AssigneeYamaguchi University
InventorsKoji Tamada, Yukimi Sakoda, Keishi Adachi
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AssigneeYamaguchi University
InventorsKoji Tamada, Yukimi Sakoda, Keishi Adachi
Estimating the term…
An object of the present invention is to provide CAR-expressing T cells that coexpress a chimeric antigen receptor (CAR) and a T cell immune function-enhancing factor and have a high immunity-inducing effect and antitumor activity, and to provide a CAR expression vector for the preparation of the CAR-expressing T cells. A CAR expression vector comprises a nucleic acid encoding a chimeric antigen receptor (CAR) and a nucleic acid encoding a T cell immune function-enhancing factor, wherein the nucleic acid encoding an immune function-enhancing factor is a nucleic acid encoding interleukin-7 and a nucleic acid encoding CCL19, a nucleic acid encoding a dominant negative mutant of SHP-1, or a nucleic acid encoding a dominant negative mutant of SHP-2, or a CAR-expressing T cell introduced with the CAR expression vector are prepared.
A CAR expression vector comprising a nucleic acid encoding a chimeric antigen receptor (CAR) and a nucleic acid encoding a T cell immune function-enhancing factor, wherein the nucleic acid encoding the T cell immune function-enhancing factor consists of a nucleic acid encoding interleukin-7 and a nucleic acid encoding CCL19.
An isolated CAR-expressing T cell, or a culture comprising the isolated CAR-expressing T cell, said isolated T cell prepared by introducing a CAR expression vector that expresses a nucleic acid encoding a CAR and a nucleic acid encoding a first T cell immune function-enhancing factor, said first T cell immune function-enhancing factor consisting of interleukin-7, and a CAR expression vector that expresses a nucleic acid encoding a CAR and a nucleic acid encoding a second T cell immune function-enhancing factor, said second T cell immune function-enhancing factor consisting of CCL19;
Dependencies are inferred from the claim text.