Compositions and methods of Aloe polysaccharides

An injectable formulation of a polymannan extract made by a method comprising:

• the steps of:
• weighing a specified quantity of a freeze-dried aloe powder, wherein the quantity is corrected for a moisture content;
• dissolving the freeze-dried aloe powder in deionized water to form a solution;
• adding a first volume of an organic solvent to the solution to form a first mixture;
• wherein the organic solvent to the deionized water ratio is at least 2.5:1;
• allowing the first mixture to settle for at least 8 hours;
• withdrawing a specified volume of a supernatant solution from the first mixture and adding a second volume of the organic solvent to the specified volume of the supernatant solution to form a second mixture;
• centrifuging the second mixture;
• observing for a presence of a precipitate in second mixture;
• adding a third volume of the organic solvent to first mixture if any precipitate is observed in second mixture;
• decanting the supernatant of the first mixture by siphoning, wherein the decantation is done only if no precipitate is observed in the second mixture;
• filtering the precipitate from the first mixture by using a filter paper and a suction funnel under vacuum;
• and recovering the powder of the polymannan extract from the suction funnel by scraping;
• wherein the freeze-dried aloe powder comprises:
• a specified quantity of very fine polymannan extract dissolved in deionized water, wherein the composition has at least 5.91% aloe polysaccharides having a molecular weight of 6.6×104, 63.85% aloe polysaccharides having a molecular weight of 4.8×105, 2.55% aloe polysaccharides having a molecular weight of 1.0×106, and 1.32% aloe polysaccharides having a molecular weight of 2.0×106, wherein the composition comprises at least 73.63% aloe polysaccharides;
• and one or more pharmaceutical preservatives.