US 10,314,295 B2 · Mouse model of hyperuricemia

Abstract

Provided is a non-human animal that is highly practical as a hyperuricenia model, the non-human animal being the following: (a) a non-human animal obtained by producing a primary chimeric non-human animal by transplantation of human hepatocytes to an immunodeficient non-human animal with liver dysfunction; and subsequently administering a purine base-containing substance to the primary chimeric non-human animal, or (b) a non-human animal obtained by producing a serially transplanted chimeric non-human animal via two steps, a first step being a step of producing a primary chimeric non-human animal by transplantation of human hepatocytes to an immunodeficient non-human animal with liver dysfunction, a second step being a step of transplanting the human hepatocytes grown in the body of the primary chimeric non-human animal to an immunodeficient non-human animal with liver dysfunction, the second step being performed one or more times; and subsequently administering a purine base-containing substance to the serially transplanted chimeric non-human animal.

Claims6

Dependent claims

A mouse model of hyperuricemia obtained by:

a) transplanting human hepatocytes into an immunodeficient mouse with liver dysfunction;
and
b) administering a diet containing about 1 to 10% by weight of an inosinic acid in a total amount of 30 g/kg body weight or more to the mouse obtained in step a) for about 7 to 28 days such that a mouse model of hyperuricemia is obtained
that has:
a liver containing 50% or more human hepatocytes,
a body weight of about 80% or more of 19.4 g,
a plasma or serum uric acid concentration of 4 mg/dL or more, and
a concentration of human albumin of at least about 3.7 mg/mL or more.

5 more elements

Dependencies are inferred from the claim text.

Mouse model of hyperuricemia

Life of this patent

Mouse model of hyperuricemia

Life of this patent

Abstract

Claims6