# US 10,312,070 B2 · Integrated sample processing for electrospray ionization devices > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,312,070 B2 - Document ID: US10312070B2 - Status: Granted - Kind: B2 - Application number: 16/170,483 - Filed: 2018-10-25 - Published: 2019-02-28 - Granted: 2019-06-04 - Earliest claimed: 2012-06-20 - Assignees: The University of North Carolina at Chapel Hill - Applicants: The University of North Carolina at Chapel Hill - Inventors: John Scott Mellors; John Michael Ramsey; Nicholas George Batz - Web page: https://patentgrep.com/patent/US10312070B2 ## CPC - H01J 49/165: {Electrospray ionisation} - G01N 27/4473: {by electric means} - G01N 27/44791: {Microapparatus (sample containers with integrated microfluidic structures B01L3/5027)} - H01J 49/0431: {for liquid samples (interfaces to liquid chromatographs G01N30/7233)} - H01J 49/0445: {with means for introducing as a spray, a jet or an aerosol (electrospray ion sources H01J49/165)} - H01J 49/26: Mass spectrometers or separator tubes - G01N 27/44743: {Introducing samples} - G01N 30/7266: {by electric field, e.g. electrospray} - H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Methods, systems and devices that generate differential axial transport in a fluidic device having at least one fluidic sample separation flow channel and at least one ESI emitter in communication with the at least one sample separation flow channel. In response to the generated differential axial transport, the at least one target analyte contained in a sample reservoir in communication with the sample separation channel is selectively transported to the at least one ESI emitter while inhibiting transport of contaminant materials contained in the sample reservoir toward the at least one ESI emitter thereby preferentially directing analyte molecules out of the at least one ESI emitter. The methods, systems and devices are particularly suitable for use with a mass spectrometer. ## Claims ### Claim 1 A method, comprising: introducing a fluid comprising a target analyte and one or more non-target components in an electrolyte medium into a separation channel of a fluidic device, wherein the separation channel is in fluid communication with an emitter located in proximity to a first end of the separation channel; introducing further electrolyte medium into a background channel of the fluidic device, wherein the background channel is in fluid communication with the separation channel through a junction located in proximity to the emitter; maintaining the fluid in the separation channel at a first effective viscosity; maintaining the electrolyte medium in the background channel at a second effective viscosity different from the first effective viscosity; and selectively transporting the target analyte toward the first end of the separation channel. ### Claim 2 (depends on 1) The method of claim 1, wherein the second effective viscosity is smaller than the first effective viscosity. ### Claim 3 (depends on 1) The method of claim 1, wherein the first effective viscosity causes the fluid in the separation channel to have a first electroosmotic mobility in the separation channel, the second effective viscosity causes the electrolyte medium to have a second electroosmotic mobility in the background channel, and wherein a difference between magnitudes of the first and second electroosmotic mobilities discharges the target analyte through the emitter. ### Claim 4 (depends on 1) The method of claim 1, wherein the separation channel comprises a first wall defining the separation channel and having a first surface charge density, and wherein the background channel comprises a second wall defining the background channel and having a second surface charge density different from the first surface charge density. ### Claim 5 (depends on 1) The method of claim 1, wherein at least one of the separation channel and the background channel comprises a first material layer on a surface of a wall defining the at least one channel. ### Claim 6 (depends on 5) The method of claim 5, wherein the first material comprises at least one material selected from the group consisting of aluminosilicate materials, mercaptopropyltrimethoxysilane materials, and polybrene-poly(vinylsulfonic acid) materials. ### Claim 7 (depends on 5) The method of claim 5, wherein the at least one of the separation channel and the background channel comprises a second material layer formed on the first material layer. ### Claim 8 (depends on 7) The method of claim 7, wherein the second material comprises poly(ethylene glycol). ### Claim 9 (depends on 1) The method of claim 1, further comprising: applying a first electrical potential to the fluid in the separation channel at a location in proximity to a second end of the separation channel opposite the first end; and applying a second electrical potential to the electrolyte medium in the background channel at a location in proximity to an end of the background channel opposite to the junction, wherein the first and second electrical potentials are selected to generate electroosmotic flow of the electrolyte medium in the background channel in a direction toward the junction, and to generate electroosmotic flow of the fluid in the separation channel in a direction away from the junction. ### Claim 10 (depends on 9) The method of claim 9, wherein the second electrical potential is positive relative to a reference ground potential. ### Claim 11 (depends on 10) The method of claim 10, wherein the first electrical potential is negative relative to the reference ground potential. ### Claim 12 (depends on 10) The method of claim 10, wherein the first electrical potential is positive relative to the reference ground potential, and wherein a magnitude of the first electrical potential is larger than a magnitude of the second electrical potential. ### Claim 13 (depends on 10) The method of claim 10, wherein an electroosmotic flow rate of the fluid in the separation channel is about 1×10−4 cm2/Vs or less. ### Claim 14 (depends on 1) The method of claim 1, further comprising: discharging the target analyte from the emitter; and maintaining the one or more non-target components in the separation channel until the target analyte has been discharged. ### Claim 15 (depends on 1) The method of claim 1, further comprising transporting the one or more non-target components in a direction away from the first end of the separation channel. ### Claim 16 A fluidic device, comprising: a separation channel formed in a substrate and comprising a first coating formed on a wall of the separation channel; a first electrode positioned so that when a fluid fills the separation channel, the first electrode contacts the fluid in the separation channel; a background channel formed in the substrate and connected to the separation channel at a junction; a second electrode positioned so that when a fluid fills the background channel, the second electrode contacts the fluid in the background channel; and an emitter connected to the separation channel, wherein the first coating is formed from a first coating material selected such that when a fluid comprising a target analyte and one or more non-target components in an electrolyte medium is introduced into the separation channel and further electrolyte medium is introduced into the background channel, the fluid in the separation channel has a first effective viscosity and the electrolyte medium in the background channel has a second effective viscosity different from the first effective viscosity; and wherein the device is configured to discharge the target analyte from the emitter and to prevent discharge of the one or more non-target components from the emitter when electrical potentials are applied to the first and second electrodes. ### Claim 17 (depends on 16) The device of claim 16, wherein the second effective viscosity is smaller than the first effective viscosity. ### Claim 18 (depends on 16) The device of claim 16, wherein the first material comprises at least one material selected from the group consisting of aluminosilicate materials, mercaptopropyltrimethoxysilane materials, and polybrene-poly(vinylsulfonic acid) materials. ### Claim 19 (depends on 16) The device of claim 16, wherein the background channel comprises a second coating formed on a wall of the background channel from a second material different from the first material. ### Claim 20 A method, comprising: disposing an electrolyte medium in a separation channel and in a background channel of a fluidic device in fluid communication with the separation channel, wherein the separation channel is configured so that an effective viscosity of the electrolyte medium in the separation channel is different from an effective viscosity of the electrolyte medium in the background channel; applying an electrical field across the electrolyte medium in the separation and background channels to cause electroosmotic flow of the electrolyte medium within the background and separation channels; transporting a target analyte from the separation channel to an emitter connected to the separation channel; and preventing transport of one or more non-target substances from the separation channel to the emitter.