# US 10,309,930 B2 · Dynamic orthogonal analysis method

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- Number: US 10,309,930 B2
- Document ID: US10309930B2
- Status: Granted
- Kind: B2
- Application number: 15/516,387
- Filed: 2015-09-21
- Published: 2017-08-31
- Granted: 2019-06-04
- Earliest claimed: 2014-10-08
- Assignees: DH Technologies Development Pte. Ltd.
- Applicants: DH Technologies Development Pte. Ltd.
- Inventors: John Lawrence Campbell; Eva Duchoslav; Yves Le Blanc; David M. Cox
- Web page: https://patentgrep.com/patent/US10309930B2

## CPC

- G01N 27/624: Differential mobility spectrometry [DMS]; Field asymmetric-waveform ion mobility spectrometry [FAIMS]
- H01J 49/0031: {Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence)}
- H01J 49/0036: {Step by step routines describing the handling of the data generated during a measurement}
- H01J 49/004: {Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn}
- H01J 49/0045: {characterised by the fragmentation or other specific reaction}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H)

## Abstract

Systems and methods are provided for providing a DMS precursor ion survey scan. An ion source configured to receive a sample is instructed to ionize the sample using a processor. A DMS device configured to receive ions from the ion source is instructed to separate precursor ions received from the ion source and transmit precursor ions using two or more CoVs using the processor. A mass analyzer configured to receive transmitted precursor ions from the DMS device is instructed to measure the m/z intensities of the transmitted precursor ions across an m/z range at each CoV of the two or more CoVs using the processor. The measured m/z intensities of the transmitted precursor ions received from the mass analyzer are stored as a function of m/z value and CoV using the processor. This produces a stored two-dimensional mapping of m/z intensities of the precursor ions of the sample.

## Claims

### Claim 1

A system for providing a differential mobility spectrometry (DMS) precursor ion survey scan, comprising:
an ion source configured to receive a sample and ionize the sample;
a differential mobility spectrometry (DMS) device configured to receive ions from the ion source and to transmit separated precursor ions based on a compensation voltage (CoV);
a mass analyzer configured to receive transmitted precursor ions from the DMS device and measure the mass-to-charge ratio (m/z) intensities of the transmitted precursor ions; and
a processor in communication with the ion source, the DMS device, and the mass analyzer that
instructs the ion source to ionize the sample,
instructs the DMS device to separate precursor ions received from the ion source and transmit precursor ions using two or more CoVs,
instructs the mass analyzer to measure the m/z intensities of the transmitted precursor ions across an m/z range at each CoV of the two or more CoVs, and
stores the measured m/z intensities of the transmitted precursor ions received from the mass analyzer as a function of m/z value and CoV, producing a two-dimensional mapping of m/z intensities of the transmitted precursor ions of the sample.

### Claim 2 (depends on 1)

The system of claim 1, wherein the processor further selects one or more regions of the stored two-dimensional mapping where m/z intensities of the transmitted precursor ions exceed a predetermined threshold for use in a data independent acquisition (DIA) tandem mass spectrometry workflow, wherein each region of the one or more regions is defined by an m/z range and a CoV range.

### Claim 3 (depends on 2)

The system of claim 2, wherein the processor further calculates a dynamic mass spectrometry/mass spectrometry (MS/MS) accumulation time for each region of the one or more region based on the m/z intensities of the transmitted precursor ions in each region and calculates an optimized collision energy for each region of the one or more region based on the m/z values of the transmitted precursor ions in each region.

### Claim 4 (depends on 1)

The system of claim 1, wherein the processor further selects each m/z intensity of each transmitted precursor ion of the stored two-dimensional mapping that exceeds a predetermined threshold and adds the m/z value and CoV of the m/z intensity that exceeds a predetermined threshold to an inclusion list for use in an information dependent acquisition (IDA) tandem mass spectrometry workflow.

### Claim 5 (depends on 4)

The system of claim 4, wherein the processor further calculates a dynamic mass spectrometry/mass spectrometry (MS/MS) accumulation time for each transmitted precursor ion of the stored two-dimensional mapping based on the m/z intensity of the each transmitted precursor ion and calculates an optimized collision energy for the each transmitted precursor ion based on the m/z values of the each transmitted precursor ion.

### Claim 6

A method for providing a differential mobility spectrometry (DMS) precursor ion survey scan, comprising:
instructing an ion source configured to receive a sample to ionize the sample using a processor;
instructing a differential mobility spectrometry (DMS) device configured to receive ions from the ion source to separate precursor ions received from the ion source and transmit precursor ions using two or more compensation voltages (CoVs) using the processor;
instructing a mass analyzer configured to receive transmitted precursor ions from the DMS device to measure the m/z intensities of the transmitted precursor ions across an m/z range at each CoV of the two or more CoVs using the processor; and
storing the measured m/z intensities of the transmitted precursor ions received from the mass analyzer as a function of m/z value and CoV, producing a two-dimensional mapping of m/z intensities of the precursor ions of the sample using the processor.

### Claim 7 (depends on 6)

The method of claim 6, further comprising selecting one or more regions of the stored two-dimensional mapping where m/z intensities of the transmitted precursor ions exceed a predetermined threshold for use in a data independent acquisition (DIA) tandem mass spectrometry workflow using the processor, wherein each region of the one or more regions is defined by an m/z range and a CoV range.

### Claim 8 (depends on 7)

The method of claim 7, further comprising calculating a dynamic mass spectrometry/mass spectrometry (MS/MS) accumulation time for each region of the one or more region based on the m/z intensities of the transmitted precursor ions in each region and calculating an optimized collision energy for each region of the one or more region based on the m/z values of the transmitted precursor ions in each region using the processor.

### Claim 9 (depends on 6)

The method of claim 6, further comprising selecting each m/z intensity of each transmitted precursor ion of the stored two-dimensional mapping that exceeds a predetermined threshold and adding the m/z value and CoV of the m/z intensity that exceeds a predetermined threshold to an inclusion list for use in an information dependent acquisition (IDA) tandem mass spectrometry workflow using the processor.

### Claim 10 (depends on 9)

The method of claim 9, further comprising calculating a dynamic mass spectrometry/mass spectrometry (MS/MS) accumulation time for each transmitted precursor ion of the stored two-dimensional mapping based on the m/z intensity of the each transmitted precursor ion and calculating an optimized collision energy for the each transmitted precursor ion based on the m/z values of the each transmitted precursor ion using the processor.

### Claim 11

A computer program product, comprising a non-transitory and tangible computer-readable storage medium whose contents include a program with instructions being executed on a processor so as to perform a method for providing a differential mobility spectrometry (DMS) precursor ion survey scan, the method comprising:
providing a system, wherein the system comprises one or more distinct software modules, and wherein the distinct software modules comprise a control module;
instructing an ion source configured to receive a sample to ionize the sample using the control module;
instructing a differential mobility spectrometry (DMS) device configured to receive ions from the ion source to separate precursor ions received from the ion source and transmit precursor ions using two or more compensation voltages (CoVs) using the control module;
instructing a mass analyzer configured to receive transmitted precursor ions from the DMS device to measure the m/z intensities of the transmitted precursor ions across an m/z range at each CoV of the two or more CoVs using the control module; and
storing the measured m/z intensities of the transmitted precursor ions received from the mass analyzer as a function of m/z value and CoV, producing a two-dimensional mapping of m/z intensities of the precursor ions of the sample using the control module.

### Claim 12 (depends on 11)

The computer program product of claim 11, further comprising selecting one or more regions of the stored two-dimensional mapping where m/z intensities of the transmitted precursor ions exceed a predetermined threshold for use in a data independent acquisition (DIA) tandem mass spectrometry workflow using the control module, wherein each region of the one or more regions is defined by an m/z range and a CoV range.

### Claim 13 (depends on 12)

The computer program product of claim 12, further comprising calculating a dynamic mass spectrometry/mass spectrometry (MS/MS) accumulation time for each region of the one or more region based on the m/z intensities of the transmitted precursor ions in each region and calculating an optimized collision energy for each region of the one or more region based on the m/z values of the transmitted precursor ions in each region using the control module.

### Claim 14 (depends on 11)

The computer program product of claim 11, further comprising selecting each m/z intensity of each transmitted precursor ion of the stored two-dimensional mapping that exceeds a predetermined threshold and adding the m/z value and CoV of the m/z intensity that exceeds a predetermined threshold to an inclusion list for use in an information dependent acquisition (IDA) tandem mass spectrometry workflow using the control module.

### Claim 15 (depends on 14)

The computer program product of claim 14, further comprising calculating a dynamic mass spectrometry/mass spectrometry (MS/MS) accumulation time for each transmitted precursor ion of the stored two-dimensional mapping based on the m/z intensity of the each transmitted precursor ion and calculating an optimized collision energy for the each transmitted precursor ion based on the m/z values of the each transmitted precursor ion using the control module.