# US 10,309,888 B2 · Biosensor comprising electrode for measuring hematocrit value > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,309,888 B2 - Document ID: US10309888B2 - Status: Granted - Kind: B2 - Application number: 15/090,649 - Filed: 2016-04-05 - Published: 2016-10-06 - Granted: 2019-06-04 - Earliest claimed: 2015-04-06 - Assignees: ARKRAY, Inc. - Applicants: ARKRAY, Inc. - Inventors: Megumi Saeda; Hisashi Kaneda - Web page: https://patentgrep.com/patent/US10309888B2 ## CPC - G01N 15/05: in blood - C12Q 1/006: {for glucose} - G01N 27/028: {Circuits therefor (measuring impedance per se G01R27/02)} - G01N 27/3272: {Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels (optical biosensors G01N33/52)} - G01N 27/3273: {Devices therefor, e.g. test element readers, circuitry (details not specific to biochemical electrodes G01N33/4875)} - G01N 27/3274: {Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration (coding of calibration information G01N33/48771)} - G01N 27/4166: {measuring a particular property of an electrolyte} - G01N 15/01: specially adapted for biological cells, e.g. blood cells (investigating sedimentation of particle suspensions in blood G01N15/05) - G01N 2015/055: {for hematocrite determination} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES ## Abstract Provided is a method for measuring the concentration of a substance in a blood sample, the method comprising the steps of: supplying the blood sample to a biosensor comprising a hematocrit electrode for measuring a hematocrit value on the surface of which electrode an electrically conductive polymer is coated by physical adsorption in the absence of a crosslinking agent; and calculating the concentration of the substance from the measured value of a first current resulting from application of a first voltage; and correcting the concentration of the substance with the value of a second current resulting from application of a second voltage or the hematocrit value calculated from the value of the second current. ## Claims ### Claim 1 A biosensor comprising: an enzyme electrode containing a detection layer, the detection layer comprising an enzyme, an electrically conductive polymer and a crosslinking agent; a counter electrode; a reference electrode; and a hematocrit electrode configured to measure a hematocrit value in a blood sample, wherein: the electrically conductive polymer is physically adsorbed as a coating on a surface of the hematocrit electrode via a non-covalent bond, the electrically conductive polymer is configured to transfer electrons received from the blood sample to the electrode, the crosslinking agent contains at least one functional group selected from the group consisting of an aldehyde group, a maleimide group, a carbodlimide group, an oxazoline group, an epoxy group and combinations thereof, and the coating does not contain a crosslinking agent. ### Claim 2 (depends on 1) The biosensor according to claim 1, wherein the electrically conductive polymer is a water-soluble electrically conductive polymer. ### Claim 3 (depends on 1) The biosensor according to claim 1, wherein the enzyme is an oxidoreductase. ### Claim 4 (depends on 1) The biosensor according to claim 1, wherein the enzyme is a saccharide oxidoreductase. ### Claim 5 (depends on 1) The biosensor according to claim 1, wherein the detection layer further comprises electrically conductive particles. ### Claim 6 (depends on 1) A measuring apparatus comprising: the biosensor according to claim 1; and a control section configured to control the application of voltage to the enzyme electrode and the hematocrit electrode of the biosensor, wherein the hematocrit value is determined from a measured value of a current which flows after a voltage is applied to the hematocrit electrode. ### Claim 7 (depends on 1) A method for measuring a hematocrit value of a blood sample, comprising: supplying the blood sample to the biosensor according to claim 1; and measuring the value of a current which flows when a voltage of 100 to 300 mV is applied to the hematocrit electrode. ### Claim 8 (depends on 1) A measuring apparatus comprising: the biosensor according to claim 1; and a control section configured to control the application of voltage to the hematocrit electrode of the biosensor, wherein the hematocrit value is determined from a measured value of a current which flows after a voltage is applied to the hematocrit electrode. ### Claim 9 (depends on 1) The biosensor according to claim 1, wherein the electrically conductive polymer is polyaniline. ### Claim 10 (depends on 9) A method for measuring the concentration of a substance in a blood sample, comprising: supplying the blood sample to the biosensor according to claim 9; measuring the value of a first current which flows when a first voltage of 0 to 400 mV is applied to the enzyme electrode, and calculating the concentration of the substance from the measured value of the first current; measuring the value of a second current which flows when a second voltage of 100 to 300 mV is applied to the hematocrit electrode; and correcting the concentration of the substance with the value of the second current or the hematocrit value calculated from the value of the second current. ### Claim 11 (depends on 10) The method according to claim 10, wherein the substance is glucose. ### Claim 12 (depends on 10) The method according to claim 10, wherein the first voltage is 100 to 200 mV and the second voltage is 150 to 250 mV.