# US 10,309,388 B2 · Continuous sample delivery peristaltic pump

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- Number: US 10,309,388 B2
- Document ID: US10309388B2
- Status: Granted
- Kind: B2
- Application number: 15/242,122
- Filed: 2016-08-19
- Published: 2017-02-23
- Granted: 2019-06-04
- Earliest claimed: 2015-08-21
- Assignees: Bio-Rad Laboratories, Inc.
- Applicants: Bio-Rad Laboratories, Inc.
- Inventors: Nathan Michael Gaskill-Fox; Daniel Nelson Fox
- Web page: https://patentgrep.com/patent/US10309388B2

## CPC

- F04B 43/1261: {the rollers being placed at the outside of the tubular flexible member}
- F04B 43/0072: {of tubular flexible members}
- F04B 43/1215: {having no backing plate (deforming of the tube only by rollers)}
- F04B 43/1276: {Means for pushing the rollers against the tubular flexible member}
- F04B: POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS (machines for liquids, or pumps, of rotary-piston or oscillating-piston type F04C; non-positive-displacement pumps F04D; pumping of fluid by direct contact of another fluid or by using inertia of fluid to be pumped F04F)

## Abstract

A method and apparatus for pumping fluid through tubing are provided. The method includes orbiting first rollers around the periphery of a first disk at a first tangential speed in a first angular sector and a slower, second tangential speed in a second angular sector, orbiting second rollers around the periphery of a second disk at the second tangential speed, and increasing the pressure of fluid in tubing between one first roller and one second roller by causing the one first roller to fully compress the tubing at the first tangential speed and simultaneously causing the one second roller to fully compress the tubing at the second tangential speed. The apparatus includes a first disk with a recess in its periphery, the first angular sector with a nominal first radius, and a second angular sector with a nominal second radius; and a second disk with the nominal first radius and a recess in its periphery.

## Claims

### Claim 1

A method of pumping a fluid through a tubing that is positioned partially around a periphery of a first disk of a peristaltic pump and partially around a periphery of a second disk of the peristaltic pump, the method comprising:
orbiting a plurality of first rollers at a constant angular speed around the periphery of the first disk such that the first rollers are constantly pressed into contact with the periphery of the first disk, the tubing, or the periphery of the first disk and the tubing, wherein the first disk includes a first angular sector that is configured to cause the first rollers to move along a first section of the periphery of the first disk at a first tangential speed and a second angular sector that is configured to cause the first rollers to move along a second section of the periphery of the first disk at a second tangential speed less than the first tangential speed;
orbiting a plurality of second rollers at the constant angular speed around the periphery of the second disk such that the second rollers are constantly pressed into contact with the periphery of the second disk, the tubing, or the periphery of the second disk and the tubing, wherein the second disk is configured to cause each second roller to move at substantially the second tangential speed;
increasing a pressure of a portion of the fluid in the tubing between one first roller and one second roller by causing the one first roller to fully compress the tubing in the first angular sector and simultaneously causing the one second roller to fully compress the tubing in a first section of the periphery of the second disk; and
moving, after increasing the pressure of the portion of the fluid, the portion of the fluid through the tubing at a constant pressure towards an output of the tubing by causing the one first roller to fully compress the tubing in the second angular sector and simultaneously causing the one second roller to fully compress the tubing.

### Claim 2 (depends on 1)

The method of claim 1, wherein:
the first disk has a first nominal radius throughout at least part of the first angular sector and a second nominal radius throughout the second angular sector,
the second disk has the second nominal radius, and
the first nominal radius is larger than the second nominal radius.

### Claim 3 (depends on 2)

The method of claim 2, wherein the first disk gradually transitions in radius from the first radius to the second radius in between the first angular sector and the second angular sector.

### Claim 4 (depends on 1)

The method of claim 1, wherein moving the portion of the fluid through the tubing at the constant pressure towards the output of the tubing further comprises:
causing, after the one first roller has moved along the second section of the periphery of the first disk:
the one first roller to move along a third angular sector of the first disk that includes a third section of the periphery of the first disk, wherein the first disk is configured to cause the one first roller to move along the third section at the second tangential speed,
the one first roller to fully compress the tubing at at least a beginning of the third section in a direction of rotation of the first roller with respect to the first disk, and
the one first roller to not fully compress the tubing at at least an end of the third section of the periphery of the first disk in the direction of rotation of the first roller with respect to the first disk; and
causing another second roller to fully compress the tubing against the second disk before causing the one first roller to not fully compress the tubing at at least the end of the third section of the periphery of the first disk.

### Claim 5 (depends on 4)

The method of claim 4, further comprising causing another first roller to fully compress the tubing before causing the one first roller to not fully compress the tubing at at least the end of the third section of the periphery of the first disk.

### Claim 6 (depends on 4)

The method of claim 4, further comprising causing the one second roller to fully compress the tubing when the one first roller is at least at the beginning of the third section of the periphery of the first disk and not to compress the tubing when the one first roller is at least at the end of the third section of the periphery of the first disk.

### Claim 7 (depends on 1)

The method of claim 1, wherein there are only two first rollers and only two second rollers.

### Claim 8 (depends on 1)

The method of claim 1, further comprising drawing fluid into the tubing through an inlet by sequentially causing each first roller to fully compress the tubing and orbit around at least part of the periphery of the first disk.

### Claim 9 (depends on 1)

The method of claim 1, wherein orbiting the plurality of first rollers at the constant angular speed around the periphery of the first disk and orbiting the plurality of second rollers at the constant angular speed around the periphery of the second disk includes fixing the first disk and the second disk in stationary positions and causing the plurality of first rollers to orbit around the first disk and causing the plurality of second rollers to orbit around the second disk.

### Claim 10 (depends on 1)

The method of claim 1, wherein the output is configured to supply the fluid to one of a flow cell or a cuvette.

### Claim 11 (depends on 1)

The method of claim 1, wherein the output of the tubing has a pressure that substantially matches the constant pressure of the portion of the fluid.

### Claim 12 (depends on 1)

The method of claim 1, wherein the output is configured to supply the fluid to a nozzle of a flow cytometer.

### Claim 13

An apparatus, comprising:
a first disk, including:
a first recess in the periphery of the first disk, the first recess configured to receive a first portion of tubing for conveying fluid,
a first angular sector that has a nominal first radius and includes a first section of a periphery of the first disk, and
a second angular sector that has a nominal second radius and includes a second section of the periphery of the first disk, wherein the second radius is smaller than the first radius, and wherein the first section of the periphery of the first disk is longer than the second section of the periphery of the first disk;
a second disk that is substantially circular, has the nominal first radius, and includes a second recess in a periphery of the second disk, the second recess configured to receive a second portion of the tubing;
a plurality of first rollers that are configured to orbit around the periphery of the first disk at a constant angular speed and configured to, when the first portion of the tubing is in the first recess, constantly press into contact with the periphery of the first disk, the tubing, or the periphery of the first disk and the tubing; and
a plurality of second rollers that are configured to orbit around the periphery of the second disk at the constant angular speed and configured to, when the second portion of the tubing is in the second recess, constantly press into contact with the periphery of the second disk, the tubing, or the periphery of the second disk and the tubing, wherein:
the first disk is configured such that each first roller moves in the first angular sector at a first tangential speed while fully compressing the tubing and such that each first roller moves in the second angular sector at a second tangential speed while fully compressing the tubing,
the second disk is configured such that each second roller moves around the periphery of the second disk at the second tangential speed,
the first disk, second disk, first rollers, and second rollers are configured to cause one first roller to fully compress the tubing while moving in the first angular sector and to simultaneously cause one second roller to fully compress the tubing while moving in a first section of the periphery of the second disk, and
the first disk, second disk, first rollers, and second rollers are further configured to cause, after the one first roller has moved past the first angular sector, the one first roller to fully compress the tubing in the second angular sector and to simultaneously cause the one second roller to fully compress the tubing.

### Claim 14 (depends on 13)

The apparatus of claim 13, wherein the first disk gradually transitions in radius from the first radius to the second radius in between the first angular sector and the second angular sector.

### Claim 15 (depends on 13)

The apparatus of claim 13, wherein:
the first disk is further configured to cause, after the one first roller has moved along the second section of the periphery of the first disk:
the one first roller to move along a third angular sector of the first disk that includes a third section of the periphery of the first disk, wherein the first disk is configured to cause the one first roller to move along the third section at the second tangential speed,
the one first roller to fully compress the tubing at at least a beginning of the third section in a direction of rotation of the first roller with respect to the first disk,
the one first roller to not fully compress the tubing at at least an end of the third section in the direction of rotation of the first roller with respect to the first disk; and
the second disk is further configured to cause another second roller to fully compress the tubing against the second disk before the one first roller is caused to not fully compress the tubing at at least the end of the third section of the periphery of the first disk.

### Claim 16 (depends on 13)

The apparatus of claim 13, further comprising:
a first roller support on which the first rollers are mounted; and
a second roller support on which the second rollers are mounted, wherein the first roller support and the second roller support are configured to rotate about a common center axis at the constant angular speed.

### Claim 17 (depends on 13)

The apparatus of claim 13, further comprising the tubing that is positioned partially around the periphery of the first disk in the first recess and positioned partially around the periphery of the second disk in the second recess.

### Claim 18 (depends on 13)

The apparatus of claim 13, wherein:
in the sections of the periphery of the first disk where the first rollers fully compress the tubing, the first recess has a first depth that is less than a nominal outer diameter of the tubing, causing the tubing to extend past the periphery of the first disk such that the first rollers fully compress the tubing, and
in the sections of the periphery of the second disk where the second rollers fully compress the tubing, the second recess has a second depth that is less than the nominal outer diameter of the tubing and that causes the tubing to extend past the periphery of the second disk such that the second rollers fully compress the tubing.

### Claim 19 (depends on 13)

The apparatus of claim 13, wherein the first disk includes a first adjustment plate, wherein the first adjustment plate is movable with respect to the remainder of the first disk such that locations along the periphery of the first disk where full compression of the tubing occurs between the first disk and a first roller are tunable.

### Claim 20 (depends on 13)

The apparatus of claim 13, wherein the second disk includes a second adjustment plate, wherein the second adjustment plate is movable with respect to the remainder of the second disk such that locations along the periphery of the second disk where full compression of the tubing occurs between the second disk and a second roller are tunable.