# US 10,308,988 B2 · Corn plant MON88017 and compositions and methods for detection thereof

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- Number: US 10,308,988 B2
- Document ID: US10308988B2
- Status: Granted
- Kind: B2
- Application number: 15/392,953
- Filed: 2016-12-28
- Published: 2017-06-15
- Granted: 2019-06-04
- Earliest claimed: 2003-12-15
- Assignees: Monsanto Technology LLC
- Applicants: Monsanto Technology LLC
- Inventors: Kim A. Beazley; Timothy R. Coombe; Mark E. Groth; Terri B. Hinchey; Jay C. Pershing; Ty T. Vaughn; Bei Zhang
- Web page: https://patentgrep.com/patent/US10308988B2

## CPC

- C12Q 1/6895: for plants, fungi or algae
- C12N 15/8275: {Glyphosate}
- C12N 15/8286: {for insect resistance}
- C12Q 2600/13: Plant traits
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The present invention provides a corn plant designated MON88017 and DNA compositions contained therein. Also provided are assays for detecting the presence of the corn plant MON88017 based on a DNA sequence and the use of this DNA sequence as a molecular marker in a DNA detection method.

## Claims

### Claim 1

A method of determining the zygosity of a corn plant comprising event MON88017 comprising:
(a) contacting a sample comprising corn DNA with a primer set that when used in a nucleic-acid amplification reaction with genomic DNA from the corn plant comprising event MON88017, produces a first amplicon that is diagnostic for corn event MON88017, and that when used in a nucleic-acid amplification reaction with genomic DNA from corn plants that do not comprise corn event MON88017 produces a second amplicon comprising the native corn genomic DNA region spanning the insertion location of the transgene of corn event MON88017;
(b) performing a nucleic acid amplification reaction, thereby producing the first amplicon or the second amplicon, or both the first and the second amplicons; and
(c) detecting the first amplicon or the second amplicon, or both the first and the second amplicons, wherein the presence of both amplicons indicates the sample is heterozygous for event MON88017 and the presence of only said first amplicon indicates said sample is homozygous for event MON88017.

### Claim 2 (depends on 1)

The method of claim 1, wherein the primer set comprises SEQ ID NO:30, SEQ ID NO:31, and SEQ ID NO:32.